TY - JOUR
T1 - Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome
AU - Armache, Jean Paul
AU - Jarasch, Alexander
AU - Anger, Andreas M.
AU - Villa, Elizabeth
AU - Becker, Thomas
AU - Bhushan, Shashi
AU - Jossinet, Fabrice
AU - Habeck, Michael
AU - Dindar, Gülcin
AU - Franckenberg, Sibylle
AU - Marquez, Viter
AU - Mielke, Thorsten
AU - Thomm, Michael
AU - Berninghausen, Otto
AU - Beatrix, Birgitta
AU - Söding, Johannes
AU - Westhof, Eric
AU - Wilson, Daniel N.
AU - Beckmann, Roland
PY - 2010/11/16
Y1 - 2010/11/16
N2 - Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectron microscopy reconstructions of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution, together with a 6.1-Å map of a translating Saccharomyces cerevisiae 80S ribosome, we have localized and modeled 74/80 (92.5%) of the ribosomal proteins, encompassing 12 archaeal/eukaryote-specific small subunit proteins as well as the complete complement of the ribosomal proteins of the eukaryotic large subunit. Near-complete atomic models of the 80S ribo- some provide insights into the structure, function, and evolution of the eukaryotic translational apparatus.
AB - Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectron microscopy reconstructions of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution, together with a 6.1-Å map of a translating Saccharomyces cerevisiae 80S ribosome, we have localized and modeled 74/80 (92.5%) of the ribosomal proteins, encompassing 12 archaeal/eukaryote-specific small subunit proteins as well as the complete complement of the ribosomal proteins of the eukaryotic large subunit. Near-complete atomic models of the 80S ribo- some provide insights into the structure, function, and evolution of the eukaryotic translational apparatus.
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U2 - 10.1073/pnas.1010005107
DO - 10.1073/pnas.1010005107
M3 - Article
C2 - 20974910
AN - SCOPUS:78650532579
SN - 0027-8424
VL - 107
SP - 19754
EP - 19759
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 46
ER -