TY - JOUR
T1 - Localization of nucleoside triphosphatase activity to the inner nuclear envelope and associated heterochromatin
AU - Clawson, Gary
AU - Friend, Daniel S.
AU - Smuckler, Edward A.
N1 - Funding Information:
This research was supported by USPHS Grants CA-21141 and AM-19843 from the NIH. authors wish to thank David Geller for his assistancei n preparingt his manuscript.
PY - 1984/11
Y1 - 1984/11
N2 - Nuclear-envelope nucleoside triphosphatase activity (NTPase), an enzymatic activity thought to participate in RNA transport, was localized in rat liver in situ after brief perfusion with 3% paraformaldehyde. Reaction product was distributed along the nucleoplasmic side of the nuclear envelope (NE) in heterochromatin, was only occasionally found at nuclear pores, and nuclear deposition was selectively blocked by inhibitors of NE NTPase activity. Our results suggest that NTPases, which are active in the NE and which participate in RNA transport, are not specifically associated with nuclear-pore complexes.
AB - Nuclear-envelope nucleoside triphosphatase activity (NTPase), an enzymatic activity thought to participate in RNA transport, was localized in rat liver in situ after brief perfusion with 3% paraformaldehyde. Reaction product was distributed along the nucleoplasmic side of the nuclear envelope (NE) in heterochromatin, was only occasionally found at nuclear pores, and nuclear deposition was selectively blocked by inhibitors of NE NTPase activity. Our results suggest that NTPases, which are active in the NE and which participate in RNA transport, are not specifically associated with nuclear-pore complexes.
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U2 - 10.1016/0014-4827(84)90796-1
DO - 10.1016/0014-4827(84)90796-1
M3 - Article
C2 - 6092120
AN - SCOPUS:0021690443
SN - 0014-4827
VL - 155
SP - 310
EP - 314
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -