Localization of sphingomyelin in cholesterol domains by imaging mass spectrometry

Carolyn M. McQuaw, Leiliang Zheng, Andrew G. Ewing, Nicholas Winograd

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


The location of each lipid in a palmitoyloleoylphosphatidylcholine/18:0 sphingomyelin/cholesterol monolayer system is laterally resolved using imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) without the necessity of adding fluorescent labels. This system of coexisting immiscible liquid phases shows cholesterol domains with sizes and shapes comparable to those in the fluorescence microscopy literature. The results show that SM localizes with cholesterol and that palmitoyloleoylphosphatidylcholine is excluded. Moreover, the segregation is not complete, and there is a small amount of both phospholipids distributed throughout.

Original languageEnglish (US)
Pages (from-to)5645-5650
Number of pages6
Issue number10
StatePublished - May 8 2007

All Science Journal Classification (ASJC) codes

  • General Materials Science
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry


Dive into the research topics of 'Localization of sphingomyelin in cholesterol domains by imaging mass spectrometry'. Together they form a unique fingerprint.

Cite this