TY - JOUR
T1 - Long-range organization of bacteriochlorophyll in chlorosomes of Chlorobium tepidum investigated by cryo-electron microscopy
AU - Oostergetel, Gert T.
AU - Reus, Michael
AU - Gomez Maqueo Chew, Aline
AU - Bryant, Donald A.
AU - Boekema, Egbert J.
AU - Holzwarth, Alfred R.
N1 - Funding Information:
We thank Mrs. Wilma Bergsma-Schutter for skillful technical assistance and Prof. Huub de Groot for discussions. E.J.B. thanks the Netherlands Organisation for Scientific Research NWO for funding of a Tecnai G2 Polara electron microscope. This work was supported by Grant DE-FG02-94ER20137 from the US Department of Energy to D.A.B.
PY - 2007/11/27
Y1 - 2007/11/27
N2 - Intact chlorosomes of Chlorobium tepidum were embedded in amorphous ice layers and examined by cryo-electron microscopy to study the long-range organization of bacteriochlorophyll (BChl) layers. End-on views reveal that chlorosomes are composed of several multi-layer tubules of variable diameter (20-30 nm) with some locally undulating non-tubular lamellae in between. The multi-layered tubular structures are more regular and larger in a C. tepidum mutant that only synthesizes [8-ethyl, 12-methyl]-BChl d. Our data show that wild-type C. tepidum chlorosomes do not have a highly regular, long-range BChl c layer organization and that they contain several multi-layered tubules rather than single-layer tubules or exclusively undulating lamellae as previously proposed.
AB - Intact chlorosomes of Chlorobium tepidum were embedded in amorphous ice layers and examined by cryo-electron microscopy to study the long-range organization of bacteriochlorophyll (BChl) layers. End-on views reveal that chlorosomes are composed of several multi-layer tubules of variable diameter (20-30 nm) with some locally undulating non-tubular lamellae in between. The multi-layered tubular structures are more regular and larger in a C. tepidum mutant that only synthesizes [8-ethyl, 12-methyl]-BChl d. Our data show that wild-type C. tepidum chlorosomes do not have a highly regular, long-range BChl c layer organization and that they contain several multi-layered tubules rather than single-layer tubules or exclusively undulating lamellae as previously proposed.
UR - http://www.scopus.com/inward/record.url?scp=36049004345&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36049004345&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2007.10.045
DO - 10.1016/j.febslet.2007.10.045
M3 - Article
C2 - 17981156
AN - SCOPUS:36049004345
SN - 0014-5793
VL - 581
SP - 5435
EP - 5439
JO - FEBS Letters
JF - FEBS Letters
IS - 28
ER -