Long-range organization of bacteriochlorophyll in chlorosomes of Chlorobium tepidum investigated by cryo-electron microscopy

Gert T. Oostergetel; Michael Reus; Aline Gomez Maqueo Chew; Donald A. Bryant; Egbert J. Boekema; Alfred R. Holzwarth

doi:10.1016/j.febslet.2007.10.045

Long-range organization of bacteriochlorophyll in chlorosomes of Chlorobium tepidum investigated by cryo-electron microscopy

Gert T. Oostergetel, Michael Reus, Aline Gomez Maqueo Chew, Donald A. Bryant, Egbert J. Boekema, Alfred R. Holzwarth

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

128 Scopus citations

Abstract

Intact chlorosomes of Chlorobium tepidum were embedded in amorphous ice layers and examined by cryo-electron microscopy to study the long-range organization of bacteriochlorophyll (BChl) layers. End-on views reveal that chlorosomes are composed of several multi-layer tubules of variable diameter (20-30 nm) with some locally undulating non-tubular lamellae in between. The multi-layered tubular structures are more regular and larger in a C. tepidum mutant that only synthesizes [8-ethyl, 12-methyl]-BChl d. Our data show that wild-type C. tepidum chlorosomes do not have a highly regular, long-range BChl c layer organization and that they contain several multi-layered tubules rather than single-layer tubules or exclusively undulating lamellae as previously proposed.

Original language	English (US)
Pages (from-to)	5435-5439
Number of pages	5
Journal	FEBS Letters
Volume	581
Issue number	28
DOIs	https://doi.org/10.1016/j.febslet.2007.10.045
State	Published - Nov 27 2007

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2007.10.045

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@article{52049bb72edd401baf9a6c0f89e53696,

title = "Long-range organization of bacteriochlorophyll in chlorosomes of Chlorobium tepidum investigated by cryo-electron microscopy",

abstract = "Intact chlorosomes of Chlorobium tepidum were embedded in amorphous ice layers and examined by cryo-electron microscopy to study the long-range organization of bacteriochlorophyll (BChl) layers. End-on views reveal that chlorosomes are composed of several multi-layer tubules of variable diameter (20-30 nm) with some locally undulating non-tubular lamellae in between. The multi-layered tubular structures are more regular and larger in a C. tepidum mutant that only synthesizes [8-ethyl, 12-methyl]-BChl d. Our data show that wild-type C. tepidum chlorosomes do not have a highly regular, long-range BChl c layer organization and that they contain several multi-layered tubules rather than single-layer tubules or exclusively undulating lamellae as previously proposed.",

author = "Oostergetel, {Gert T.} and Michael Reus and {Gomez Maqueo Chew}, Aline and Bryant, {Donald A.} and Boekema, {Egbert J.} and Holzwarth, {Alfred R.}",

note = "Funding Information: We thank Mrs. Wilma Bergsma-Schutter for skillful technical assistance and Prof. Huub de Groot for discussions. E.J.B. thanks the Netherlands Organisation for Scientific Research NWO for funding of a Tecnai G2 Polara electron microscope. This work was supported by Grant DE-FG02-94ER20137 from the US Department of Energy to D.A.B.",

year = "2007",

month = nov,

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doi = "10.1016/j.febslet.2007.10.045",

language = "English (US)",

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pages = "5435--5439",

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T1 - Long-range organization of bacteriochlorophyll in chlorosomes of Chlorobium tepidum investigated by cryo-electron microscopy

AU - Oostergetel, Gert T.

AU - Reus, Michael

AU - Gomez Maqueo Chew, Aline

AU - Bryant, Donald A.

AU - Boekema, Egbert J.

AU - Holzwarth, Alfred R.

N1 - Funding Information: We thank Mrs. Wilma Bergsma-Schutter for skillful technical assistance and Prof. Huub de Groot for discussions. E.J.B. thanks the Netherlands Organisation for Scientific Research NWO for funding of a Tecnai G2 Polara electron microscope. This work was supported by Grant DE-FG02-94ER20137 from the US Department of Energy to D.A.B.

PY - 2007/11/27

Y1 - 2007/11/27

N2 - Intact chlorosomes of Chlorobium tepidum were embedded in amorphous ice layers and examined by cryo-electron microscopy to study the long-range organization of bacteriochlorophyll (BChl) layers. End-on views reveal that chlorosomes are composed of several multi-layer tubules of variable diameter (20-30 nm) with some locally undulating non-tubular lamellae in between. The multi-layered tubular structures are more regular and larger in a C. tepidum mutant that only synthesizes [8-ethyl, 12-methyl]-BChl d. Our data show that wild-type C. tepidum chlorosomes do not have a highly regular, long-range BChl c layer organization and that they contain several multi-layered tubules rather than single-layer tubules or exclusively undulating lamellae as previously proposed.

AB - Intact chlorosomes of Chlorobium tepidum were embedded in amorphous ice layers and examined by cryo-electron microscopy to study the long-range organization of bacteriochlorophyll (BChl) layers. End-on views reveal that chlorosomes are composed of several multi-layer tubules of variable diameter (20-30 nm) with some locally undulating non-tubular lamellae in between. The multi-layered tubular structures are more regular and larger in a C. tepidum mutant that only synthesizes [8-ethyl, 12-methyl]-BChl d. Our data show that wild-type C. tepidum chlorosomes do not have a highly regular, long-range BChl c layer organization and that they contain several multi-layered tubules rather than single-layer tubules or exclusively undulating lamellae as previously proposed.

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Long-range organization of bacteriochlorophyll in chlorosomes of Chlorobium tepidum investigated by cryo-electron microscopy

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