Long-read genome assembly and genetic architecture of fruit shape in the bottle gourd

Pei Xu, Ying Wang, Fengshuo Sun, Rongling Wu, Huilong Du, Yuhong Wang, Libo Jiang, Xiaohua Wu, Xinyi Wu, Liming Yang, Nailin Xing, Yaowen Hu, Baogen Wang, Yunping Huang, Ye Tao, Qiang Gao, Chengzhi Liang, Yanwei Li, Zhongfu Lu, Guojing Li

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


The bottle gourd (Lagenaria siceraria, Cucurbitaceae) is an important horticultural crop exhibiting tremendous diversity in fruit shape. The genetic architecture of fruit shape variation in this species remains unknown. We assembled a long-read-based, high-quality reference genome (ZAAS_Lsic_2.0) with a contig N50 value over 390-fold greater than the existing reference genomes. We then focused on dissection of fruit shape using a one-step geometric morphometrics-based functional mapping approach. We identified 11 quantitative trait loci (QTLs) responsible for fruit shape (fsQTLs), reconstructed their visible effects and revealed syntenic relationships of bottle gourd fsQTLs with 12 fsQTLs previously reported in cucumber, melon or watermelon. Homologs of several well-known and newly identified fruit shape genes, including SUN, OFP, AP2 and auxin transporters, were comapped with bottle gourd QTLs.

Original languageEnglish (US)
Pages (from-to)956-968
Number of pages13
JournalPlant Journal
Issue number3
StatePublished - Aug 2021

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology


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