TY - JOUR
T1 - LUCA-15 suppresses CD95-mediated apoptosis in Jurkat T cells
AU - Sutherland, Leslie C.
AU - Lerman, Michael
AU - Williams, Gwyn T.
AU - Miller, Barbara A.
N1 - Funding Information:
The authors thank Thomas Rothstein for useful advice and critical reading of the manuscript, Ann Stanley for densitometric analysis, and Marty Hansell for graphical assistance. Funding was provided by a PennState Geisinger Cancer Center Research Grant (to LC Sutherland), Contract No. NO1-CO-56000 from the National Cancer Institute, National Institutes of Health (to M Lerman), and the National Institutes of Health DK46778 and M01 RR10732, and the Four Diamonds Fund (to BA Miller). The content of the publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government.
PY - 2001/5/10
Y1 - 2001/5/10
N2 - The candidate tumour suppressor gene, LUCA-15, maps to the lung cancer tumour suppressor locus 3p21.3. Overexpression of an alternative RNA splice variant of LUCA-15 has been shown to retard human Jurkat T cell proliferation and to accelerate CD95-mediated apoptosis. An antisense cDNA to the 3′-UTR of this splice variant was able to suppress CD95-mediated apoptosis. Here, we report that overexpression of LUCA-15 itself suppresses CD95-mediated apoptosis in Jurkat cells. This suppression occurs prior to the final execution stage of the CD95 signalling pathway, and is associated with upregulation of the apoptosis inhibitory protein Bcl-2. LUCA-15 overexpression is also able to inhibit apoptosis induced by the protein kinase inhibitor staurosporine, but is not able to significantly suppress apoptosis mediated by the topoisomerase II inhibitor etoposide. These findings suggest that LUCA-15 is a selective inhibitor of cell death, and confirm the importance of the LUCA-15 genetic locus in the control of apoptosis.
AB - The candidate tumour suppressor gene, LUCA-15, maps to the lung cancer tumour suppressor locus 3p21.3. Overexpression of an alternative RNA splice variant of LUCA-15 has been shown to retard human Jurkat T cell proliferation and to accelerate CD95-mediated apoptosis. An antisense cDNA to the 3′-UTR of this splice variant was able to suppress CD95-mediated apoptosis. Here, we report that overexpression of LUCA-15 itself suppresses CD95-mediated apoptosis in Jurkat cells. This suppression occurs prior to the final execution stage of the CD95 signalling pathway, and is associated with upregulation of the apoptosis inhibitory protein Bcl-2. LUCA-15 overexpression is also able to inhibit apoptosis induced by the protein kinase inhibitor staurosporine, but is not able to significantly suppress apoptosis mediated by the topoisomerase II inhibitor etoposide. These findings suggest that LUCA-15 is a selective inhibitor of cell death, and confirm the importance of the LUCA-15 genetic locus in the control of apoptosis.
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U2 - 10.1038/sj.onc.1204371
DO - 10.1038/sj.onc.1204371
M3 - Article
C2 - 11420683
AN - SCOPUS:0035837349
SN - 0950-9232
VL - 20
SP - 2713
EP - 2719
JO - Oncogene
JF - Oncogene
IS - 21
ER -
