Lymphocyte subsets in hemophilic patients with hepatitis C virus infection with or without human immunodeficiency virus co-infection: A nested cross-sectional study

Mingdong Zhang; Thomas R. O'Brien; William C. Kopp; James J. Goedert; P. S. Rosenberg; C. S. Rabkin; E. A. Engels; D. Whitby; M. E. Eyster; B. Konkle; M. Manco-Johnson; D. DiMichele; M. W. Hilgartner; P. Blatt; L. M. Aledort; S. Seremetes; K. Hoots; A. L. Angiolillo; N. L.C. Luban; A. Cohen; C. S. Manno; C. Leissinger; G. C. White; M. M. Lederman; S. Purvis; J. Salkowitz; C. M. Kessler; A. Karafoulidou; T. Mandalaki; A. Hatzakis; G. Touloumi; W. Schramm; F. Rommel; P. de Moerloose; S. Eichinger; K. E. Sherman; B. L. Kroner

doi:10.1186/1471-2326-5-2

Lymphocyte subsets in hemophilic patients with hepatitis C virus infection with or without human immunodeficiency virus co-infection: A nested cross-sectional study

Mingdong Zhang, Thomas R. O'Brien, William C. Kopp, James J. Goedert, P. S. Rosenberg, C. S. Rabkin, E. A. Engels, D. Whitby, M. E. Eyster, B. Konkle, M. Manco-Johnson, D. DiMichele, M. W. Hilgartner, P. Blatt, L. M. Aledort, S. Seremetes, K. Hoots, A. L. Angiolillo, N. L.C. Luban, A. CohenC. S. Manno, C. Leissinger, G. C. White, M. M. Lederman, S. Purvis, J. Salkowitz, C. M. Kessler, A. Karafoulidou, T. Mandalaki, A. Hatzakis, G. Touloumi, W. Schramm, F. Rommel, P. de Moerloose, S. Eichinger, K. E. Sherman, B. L. Kroner

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Abstract

Background: With chronic infection, hepatitis C virus (HCV) RNA can be detected in B cells and associated with B-cell disorders, but these are not well defined. Methods: The relationship between HCV infection and lymphocyte subpopulations was evaluated rigorously in 120 asymptomatic hemophilic patients, randomly selected from a prospective cohort study. CD4⁺ T cells, CD8⁺ T cells, CD19⁺ B cells, and CD56⁺ NK cells were quantified by flow cytometry using cryopreserved peripheral blood mononuclear cells of 24 hemophilic patients in each of five age-matched groups [uninfected; chronic HCV with or without human immunodeficiency virus (HIV); and cleared HCV with or without HIV]. Results: As expected, patients with HIV had significantly reduced CD4⁺ and increased CD8⁺ T cells. Irrespective of HIV, patients with chronic HCV infection had approximately 25% fewer CD19⁺ B cells than those without chronic HCV infection. Conclusions: These data support the hypothesis that asymptomatic patients with chronic HCV infection have an altered B-lymphocyte population.

Original language	English (US)
Article number	2
Journal	BMC Blood Disorders
Volume	5
DOIs	https://doi.org/10.1186/1471-2326-5-2
State	Published - Jan 18 2005

All Science Journal Classification (ASJC) codes

Hematology
Molecular Biology

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10.1186/1471-2326-5-2

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Zhang, M., O'Brien, T. R., Kopp, W. C., Goedert, J. J., Rosenberg, P. S., Rabkin, C. S., Engels, E. A., Whitby, D., Eyster, M. E., Konkle, B., Manco-Johnson, M., DiMichele, D., Hilgartner, M. W., Blatt, P., Aledort, L. M., Seremetes, S., Hoots, K., Angiolillo, A. L., Luban, N. L. C., ... Kroner, B. L. (2005). Lymphocyte subsets in hemophilic patients with hepatitis C virus infection with or without human immunodeficiency virus co-infection: A nested cross-sectional study. BMC Blood Disorders, 5, Article 2. https://doi.org/10.1186/1471-2326-5-2

@article{0c6609ac29c045b2b90c321715d9a9c1,

title = "Lymphocyte subsets in hemophilic patients with hepatitis C virus infection with or without human immunodeficiency virus co-infection: A nested cross-sectional study",

abstract = "Background: With chronic infection, hepatitis C virus (HCV) RNA can be detected in B cells and associated with B-cell disorders, but these are not well defined. Methods: The relationship between HCV infection and lymphocyte subpopulations was evaluated rigorously in 120 asymptomatic hemophilic patients, randomly selected from a prospective cohort study. CD4+ T cells, CD8+ T cells, CD19+ B cells, and CD56+ NK cells were quantified by flow cytometry using cryopreserved peripheral blood mononuclear cells of 24 hemophilic patients in each of five age-matched groups [uninfected; chronic HCV with or without human immunodeficiency virus (HIV); and cleared HCV with or without HIV]. Results: As expected, patients with HIV had significantly reduced CD4+ and increased CD8+ T cells. Irrespective of HIV, patients with chronic HCV infection had approximately 25% fewer CD19+ B cells than those without chronic HCV infection. Conclusions: These data support the hypothesis that asymptomatic patients with chronic HCV infection have an altered B-lymphocyte population.",

author = "Mingdong Zhang and O'Brien, {Thomas R.} and Kopp, {William C.} and Goedert, {James J.} and Rosenberg, {P. S.} and Rabkin, {C. S.} and Engels, {E. A.} and D. Whitby and Eyster, {M. E.} and B. Konkle and M. Manco-Johnson and D. DiMichele and Hilgartner, {M. W.} and P. Blatt and Aledort, {L. M.} and S. Seremetes and K. Hoots and Angiolillo, {A. L.} and Luban, {N. L.C.} and A. Cohen and Manno, {C. S.} and C. Leissinger and White, {G. C.} and Lederman, {M. M.} and S. Purvis and J. Salkowitz and Kessler, {C. M.} and A. Karafoulidou and T. Mandalaki and A. Hatzakis and G. Touloumi and W. Schramm and F. Rommel and {de Moerloose}, P. and S. Eichinger and Sherman, {K. E.} and Kroner, {B. L.}",

year = "2005",

month = jan,

day = "18",

doi = "10.1186/1471-2326-5-2",

language = "English (US)",

volume = "5",

journal = "BMC Blood Disorders",

issn = "1471-2326",

publisher = "BioMed Central",

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Zhang, M, O'Brien, TR, Kopp, WC, Goedert, JJ, Rosenberg, PS, Rabkin, CS, Engels, EA, Whitby, D, Eyster, ME, Konkle, B, Manco-Johnson, M, DiMichele, D, Hilgartner, MW, Blatt, P, Aledort, LM, Seremetes, S, Hoots, K, Angiolillo, AL, Luban, NLC, Cohen, A, Manno, CS, Leissinger, C, White, GC, Lederman, MM, Purvis, S, Salkowitz, J, Kessler, CM, Karafoulidou, A, Mandalaki, T, Hatzakis, A, Touloumi, G, Schramm, W, Rommel, F, de Moerloose, P, Eichinger, S, Sherman, KE & Kroner, BL 2005, 'Lymphocyte subsets in hemophilic patients with hepatitis C virus infection with or without human immunodeficiency virus co-infection: A nested cross-sectional study', BMC Blood Disorders, vol. 5, 2. https://doi.org/10.1186/1471-2326-5-2

TY - JOUR

T1 - Lymphocyte subsets in hemophilic patients with hepatitis C virus infection with or without human immunodeficiency virus co-infection

T2 - A nested cross-sectional study

AU - Zhang, Mingdong

AU - O'Brien, Thomas R.

AU - Kopp, William C.

AU - Goedert, James J.

AU - Rosenberg, P. S.

AU - Rabkin, C. S.

AU - Engels, E. A.

AU - Whitby, D.

AU - Eyster, M. E.

AU - Konkle, B.

AU - Manco-Johnson, M.

AU - DiMichele, D.

AU - Hilgartner, M. W.

AU - Blatt, P.

AU - Aledort, L. M.

AU - Seremetes, S.

AU - Hoots, K.

AU - Angiolillo, A. L.

AU - Luban, N. L.C.

AU - Cohen, A.

AU - Manno, C. S.

AU - Leissinger, C.

AU - White, G. C.

AU - Lederman, M. M.

AU - Purvis, S.

AU - Salkowitz, J.

AU - Kessler, C. M.

AU - Karafoulidou, A.

AU - Mandalaki, T.

AU - Hatzakis, A.

AU - Touloumi, G.

AU - Schramm, W.

AU - Rommel, F.

AU - de Moerloose, P.

AU - Eichinger, S.

AU - Sherman, K. E.

AU - Kroner, B. L.

PY - 2005/1/18

Y1 - 2005/1/18

N2 - Background: With chronic infection, hepatitis C virus (HCV) RNA can be detected in B cells and associated with B-cell disorders, but these are not well defined. Methods: The relationship between HCV infection and lymphocyte subpopulations was evaluated rigorously in 120 asymptomatic hemophilic patients, randomly selected from a prospective cohort study. CD4+ T cells, CD8+ T cells, CD19+ B cells, and CD56+ NK cells were quantified by flow cytometry using cryopreserved peripheral blood mononuclear cells of 24 hemophilic patients in each of five age-matched groups [uninfected; chronic HCV with or without human immunodeficiency virus (HIV); and cleared HCV with or without HIV]. Results: As expected, patients with HIV had significantly reduced CD4+ and increased CD8+ T cells. Irrespective of HIV, patients with chronic HCV infection had approximately 25% fewer CD19+ B cells than those without chronic HCV infection. Conclusions: These data support the hypothesis that asymptomatic patients with chronic HCV infection have an altered B-lymphocyte population.

AB - Background: With chronic infection, hepatitis C virus (HCV) RNA can be detected in B cells and associated with B-cell disorders, but these are not well defined. Methods: The relationship between HCV infection and lymphocyte subpopulations was evaluated rigorously in 120 asymptomatic hemophilic patients, randomly selected from a prospective cohort study. CD4+ T cells, CD8+ T cells, CD19+ B cells, and CD56+ NK cells were quantified by flow cytometry using cryopreserved peripheral blood mononuclear cells of 24 hemophilic patients in each of five age-matched groups [uninfected; chronic HCV with or without human immunodeficiency virus (HIV); and cleared HCV with or without HIV]. Results: As expected, patients with HIV had significantly reduced CD4+ and increased CD8+ T cells. Irrespective of HIV, patients with chronic HCV infection had approximately 25% fewer CD19+ B cells than those without chronic HCV infection. Conclusions: These data support the hypothesis that asymptomatic patients with chronic HCV infection have an altered B-lymphocyte population.

UR - http://www.scopus.com/inward/record.url?scp=13644250284&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13644250284&partnerID=8YFLogxK

U2 - 10.1186/1471-2326-5-2

DO - 10.1186/1471-2326-5-2

M3 - Article

C2 - 15656905

AN - SCOPUS:13644250284

SN - 1471-2326

VL - 5

JO - BMC Blood Disorders

JF - BMC Blood Disorders

M1 - 2

ER -

Lymphocyte subsets in hemophilic patients with hepatitis C virus infection with or without human immunodeficiency virus co-infection: A nested cross-sectional study

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