Macro-asparatate aminotransferase in a female with antibodies to hepatitis C virus

John Collins, Detlef Ritter, Bruce R. Bacon, Michael Landt, Michael Creer

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Persistent elevation of aspartate aminotransferase (AST) activity in serum due to the presence of a macroenzyme form of AST (macro-AST) may lead to diagnostic confusion in many clinical conditions, particularly those associated with chronic liver disease. We describe a case of macro-AST arising in an adult female with a false-positive hepatitis C virus (HCV) RNA test result that was not accompanied by other biochemical or histologic evidence of liver disease. The presence of macro-AST in serum was confirmed utilizing size-exclusion, high performance liquid chromatography (HPLC) and Protein G-agarose beads to precipitate immune complexes of AST and immunoglobulin G followed by centrifugation and AST activity measurements in the supernatant. A brief review of the clinical enzymology of AST and methods used to quantify serum macro-AST activity is provided.

Original languageEnglish (US)
Pages (from-to)501-506
Number of pages6
Issue number6
StatePublished - Dec 2002

All Science Journal Classification (ASJC) codes

  • Hepatology


Dive into the research topics of 'Macro-asparatate aminotransferase in a female with antibodies to hepatitis C virus'. Together they form a unique fingerprint.

Cite this