TY - JOUR
T1 - Macrophage migration inhibitory factor in the bovine corpus luteum
T2 - Characterization of steady-state messenger ribonucleic acid and immunohistochemical localization
AU - Bove, Susan E.
AU - Petroff, Margaret G.
AU - Nishibori, Masahiro
AU - Pate, Joy L.
PY - 2000
Y1 - 2000
N2 - Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine produced by T cells and macrophages. A number of tissues also produce MIF during states of active differentiation and/or proliferation. The purpose of this study was to determine whether MIF is present in the corpus luteum (CL). The steady-state mRNA for MIF was examined in CL by Northern analysis on Day 5, Days 9-12, and Day 18 of the estrous cycle and at 0.5, 1, 4, 12, 24, and 36 h after a luteolytic injection of prostaglandin F(2α) (PGF(2α)) (n = 4 CL per time point). The greatest amount of MIF mRNA was observed in Day 5 CL compared with midcycle and Day 18 CL. Messenger RNA for MIF in CL collected 0.5 h post-PGF(2α) was greater than in midcycle and all other regressing CL. Immunohistochemical analysis (n = 4) revealed that MIF was present in the bovine CL throughout the estrous cycle and appeared to be localized to large luteal cells. It was concluded that MIF is produced within the bovine CL, mRNA expression is maximal in the early CL, and the protein is primarily localized to large luteal cells. The functional significance of MIF remains to be determined.
AB - Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine produced by T cells and macrophages. A number of tissues also produce MIF during states of active differentiation and/or proliferation. The purpose of this study was to determine whether MIF is present in the corpus luteum (CL). The steady-state mRNA for MIF was examined in CL by Northern analysis on Day 5, Days 9-12, and Day 18 of the estrous cycle and at 0.5, 1, 4, 12, 24, and 36 h after a luteolytic injection of prostaglandin F(2α) (PGF(2α)) (n = 4 CL per time point). The greatest amount of MIF mRNA was observed in Day 5 CL compared with midcycle and Day 18 CL. Messenger RNA for MIF in CL collected 0.5 h post-PGF(2α) was greater than in midcycle and all other regressing CL. Immunohistochemical analysis (n = 4) revealed that MIF was present in the bovine CL throughout the estrous cycle and appeared to be localized to large luteal cells. It was concluded that MIF is produced within the bovine CL, mRNA expression is maximal in the early CL, and the protein is primarily localized to large luteal cells. The functional significance of MIF remains to be determined.
UR - http://www.scopus.com/inward/record.url?scp=0034098796&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034098796&partnerID=8YFLogxK
U2 - 10.1095/biolreprod62.4.879
DO - 10.1095/biolreprod62.4.879
M3 - Article
C2 - 10727256
AN - SCOPUS:0034098796
SN - 0006-3363
VL - 62
SP - 879
EP - 885
JO - Biology of reproduction
JF - Biology of reproduction
IS - 4
ER -