Magnetic resonance studies of the anomeric distribution and manganese binding properties of fructose phosphates

Stephen J. Benkovic; James L. Engle; Albert S. Mildvan

doi:10.1016/0006-291X(72)90571-2

Magnetic resonance studies of the anomeric distribution and manganese binding properties of fructose phosphates

Stephen J. Benkovic, James L. Engle, Albert S. Mildvan

Chemistry

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30 Scopus citations

Abstract

The anomeric distributions of the furanose forms of fructose-6-P and fructose-1,6-diP have been found by ¹³C NMR to be 20 ± 4% α and 80 ± 10% β. The dissociation constants of the Mn complexes of fructose-1,6-diP and of a configurationally stable cis-1,6-diP analog are similar to those of various Mn-monophosphate complexes and are therefore inconsistent with chelation of the Mn by the cis-diphosphoryl moieties. Thus the affinity for metal is an unsatisfactory method for diagnosis of anomeric equilibria.

Original language	English (US)
Pages (from-to)	852-858
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	47
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(72)90571-2
State	Published - May 26 1972

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Magnetic resonance studies of the anomeric distribution and manganese binding properties of fructose phosphates",

abstract = "The anomeric distributions of the furanose forms of fructose-6-P and fructose-1,6-diP have been found by 13C NMR to be 20 ± 4% α and 80 ± 10% β. The dissociation constants of the Mn complexes of fructose-1,6-diP and of a configurationally stable cis-1,6-diP analog are similar to those of various Mn-monophosphate complexes and are therefore inconsistent with chelation of the Mn by the cis-diphosphoryl moieties. Thus the affinity for metal is an unsatisfactory method for diagnosis of anomeric equilibria.",

author = "Benkovic, {Stephen J.} and Engle, {James L.} and Mildvan, {Albert S.}",

note = "Funding Information: by USPHS Grant and by grants and RR-05539",

year = "1972",

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doi = "10.1016/0006-291X(72)90571-2",

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T1 - Magnetic resonance studies of the anomeric distribution and manganese binding properties of fructose phosphates

AU - Benkovic, Stephen J.

AU - Engle, James L.

AU - Mildvan, Albert S.

N1 - Funding Information: by USPHS Grant and by grants and RR-05539

PY - 1972/5/26

Y1 - 1972/5/26

N2 - The anomeric distributions of the furanose forms of fructose-6-P and fructose-1,6-diP have been found by 13C NMR to be 20 ± 4% α and 80 ± 10% β. The dissociation constants of the Mn complexes of fructose-1,6-diP and of a configurationally stable cis-1,6-diP analog are similar to those of various Mn-monophosphate complexes and are therefore inconsistent with chelation of the Mn by the cis-diphosphoryl moieties. Thus the affinity for metal is an unsatisfactory method for diagnosis of anomeric equilibria.

AB - The anomeric distributions of the furanose forms of fructose-6-P and fructose-1,6-diP have been found by 13C NMR to be 20 ± 4% α and 80 ± 10% β. The dissociation constants of the Mn complexes of fructose-1,6-diP and of a configurationally stable cis-1,6-diP analog are similar to those of various Mn-monophosphate complexes and are therefore inconsistent with chelation of the Mn by the cis-diphosphoryl moieties. Thus the affinity for metal is an unsatisfactory method for diagnosis of anomeric equilibria.

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Magnetic resonance studies of the anomeric distribution and manganese binding properties of fructose phosphates

Abstract

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