"Making it Real" Time

Kent E. Vrana

doi:10.2144/000114042

"Making it Real" Time

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Abstract

It has been 30 years since the first description of the polymerase chain reaction (PCR)-corresponding, coincidentally, with the year of the first issue of BioTechniques. Ensuing decades have seen remarkable advances in this revolutionary technique. Central to these approaches was the creation of paradigms for monitoring the progress of PCR amplification in real time. One of these seminal reports appeared in BioTechniques in 1997, establishing the double-strand-specific dye SYBR Green as a workhorse tool for continuous monitoring of DNA amplification.

Original language	English (US)
Pages (from-to)	312-313
Number of pages	2
Journal	BioTechniques
Volume	54
Issue number	6
DOIs	https://doi.org/10.2144/000114042
State	Published - Jun 2013

All Science Journal Classification (ASJC) codes

Biotechnology
Biochemistry, Genetics and Molecular Biology(all)

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10.2144/000114042

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abstract = "It has been 30 years since the first description of the polymerase chain reaction (PCR)-corresponding, coincidentally, with the year of the first issue of BioTechniques. Ensuing decades have seen remarkable advances in this revolutionary technique. Central to these approaches was the creation of paradigms for monitoring the progress of PCR amplification in real time. One of these seminal reports appeared in BioTechniques in 1997, establishing the double-strand-specific dye SYBR Green as a workhorse tool for continuous monitoring of DNA amplification.",

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"Making it Real" Time

Abstract

All Science Journal Classification (ASJC) codes

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