TY - JOUR
T1 - Making it stick
T2 - A CURE designed to introduce students to the scientific process and the host response to foreign materials
AU - Slee, Joshua B.
AU - McLaughlin, Jacqueline S.
N1 - Funding Information:
The development of this CURE was supported by the American Society of Cell Biology Mentoring in Active Learning and Teaching Fellowship to Joshua B. Slee. As part of this fellowship, Jacqueline S. McLaughlin served as the mentor in the development of this CURE. Additional support provided by the DeSales University Department of Natural Science and the Penn State University, Lehigh Valley Department of Biology. The authors express their gratitude to Dr. Stanley J. Stachelek of The Children’s Hospital of Philadelphia for providing the polyurethane films used in this CURE and Heather Amatore and Marjory J. Nelcoski for critical manuscript editing.
Funding Information:
The development of this CURE was supported by the American Society of Cell Biology Mentoring in Active Learning and Teaching Fellowship to Joshua B. Slee. As part of this fellowship, Jacqueline S. McLaughlin served as the mentor in the development of this CURE. Additional support provided by the DeSales University Department of Natural Science and the Penn State University, Lehigh Valley Department of Biology. The authors express their gratitude to Dr. Stanley J. Stachelek of The Children's Hospital of Philadelphia for providing the polyurethane films used in this CURE and Heather Amatore and Marjory J. Nelcoski for critical manuscript editing.
Publisher Copyright:
© 2019 International Union of Biochemistry and Molecular Biology
PY - 2019/7/1
Y1 - 2019/7/1
N2 - Often overlooked in many molecular cell biology laboratory and cell culture courses, suspension cells represent an important aspect of molecular cell biology and cell culture. Most primary cell cultures and cell lines are adherent cells which grow in monolayers on surfaces. However, other cells such as hematopoietic cells, certain tumor cells, and cells of the immune system are suspension cells which are anchorage-independent which grow and divide in solution. THP-1 cells are a commercially available, spontaneously immortalized monocyte-like cell line derived from the peripheral blood of a patient with acute monocytic leukemia. These cells are an excellent model for suspension cell culture and studies of the immune system. Researchers have used THP-1 cells to study the host response to implantable devices and biomaterials in vitro. Tissue contacting surfaces of implantable materials initiate a host inflammatory response characterized by many events, one of which includes macrophage attachment to the surface, which ultimately leads to degradation and failure of the material. Using the THP-1 adhesion assay embedded in this CURE, students can participate in the scientific process by testing substances which may prevent the host inflammatory response to implantable devices and biomaterials.
AB - Often overlooked in many molecular cell biology laboratory and cell culture courses, suspension cells represent an important aspect of molecular cell biology and cell culture. Most primary cell cultures and cell lines are adherent cells which grow in monolayers on surfaces. However, other cells such as hematopoietic cells, certain tumor cells, and cells of the immune system are suspension cells which are anchorage-independent which grow and divide in solution. THP-1 cells are a commercially available, spontaneously immortalized monocyte-like cell line derived from the peripheral blood of a patient with acute monocytic leukemia. These cells are an excellent model for suspension cell culture and studies of the immune system. Researchers have used THP-1 cells to study the host response to implantable devices and biomaterials in vitro. Tissue contacting surfaces of implantable materials initiate a host inflammatory response characterized by many events, one of which includes macrophage attachment to the surface, which ultimately leads to degradation and failure of the material. Using the THP-1 adhesion assay embedded in this CURE, students can participate in the scientific process by testing substances which may prevent the host inflammatory response to implantable devices and biomaterials.
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U2 - 10.1002/bmb.21248
DO - 10.1002/bmb.21248
M3 - Article
C2 - 31002463
AN - SCOPUS:85064674575
SN - 1470-8175
VL - 47
SP - 417
EP - 425
JO - Biochemistry and Molecular Biology Education
JF - Biochemistry and Molecular Biology Education
IS - 4
ER -