TY - JOUR
T1 - Manipulating Agaricus bisporus developmental patterns by passaging microbial communities in complex substrates
AU - Vieira, Fabricio Rocha
AU - Tomassi, Isako Di
AU - O'Connor, Eoin
AU - Bull, Carolee T.
AU - Pecchia, John A.
AU - Hockett, Kevin L.
N1 - Publisher Copyright:
Copyright © 2023 Vieira et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
PY - 2023/12
Y1 - 2023/12
N2 - There is growing interest in microbiome-based management approaches for plant and animal health and sustainable agricultural practices. To our knowledge, there are no descriptions of Agaricus bisporus mushroom developmental patterns using such an approach. Here, we utilized substrate passaging to select for devomes (developmental microbiomes) in either compost or casing material that resulted in earlier developing (best performing experimental units with a higher number of primordia at day 10 of cultivation) mushrooms compared to unpassaged controls. Passaged casing showed earlier pinning (fruiting body formation), while passaged compost delayed fruiting body formation. Despite changes in pin emergence for passaged substrates and higher mushroom yield in the passaged casing’s first harvest, the total mushroom yield in 24 days of crop cycle did not change significantly. The bacterial communities were distinct between compost and casing materials, but in both microenvironments, the alpha diversity tended to increase and stabilize over time in standard and passaged substrates. Community composition within compost and casing microenvironments tended to be more homogenous after colonization of the fungus. Passaging of casing increased the bacterial alpha diversity and shifted community composition. Such changes were not observed in passaged compost. Some bacterial taxa were enriched or depleted by passaging, e.g., Pseudomonas spp. (hypothesized as a stimulus to A. bisporus fructification) were depleted in passaged compost and casing. Overall, this work presents an experimental approach to manipulate compost and casing microbial communities with the potential to be combined with currently employed cultivation strategies toward a more sustainable mushroom cultivation system.
AB - There is growing interest in microbiome-based management approaches for plant and animal health and sustainable agricultural practices. To our knowledge, there are no descriptions of Agaricus bisporus mushroom developmental patterns using such an approach. Here, we utilized substrate passaging to select for devomes (developmental microbiomes) in either compost or casing material that resulted in earlier developing (best performing experimental units with a higher number of primordia at day 10 of cultivation) mushrooms compared to unpassaged controls. Passaged casing showed earlier pinning (fruiting body formation), while passaged compost delayed fruiting body formation. Despite changes in pin emergence for passaged substrates and higher mushroom yield in the passaged casing’s first harvest, the total mushroom yield in 24 days of crop cycle did not change significantly. The bacterial communities were distinct between compost and casing materials, but in both microenvironments, the alpha diversity tended to increase and stabilize over time in standard and passaged substrates. Community composition within compost and casing microenvironments tended to be more homogenous after colonization of the fungus. Passaging of casing increased the bacterial alpha diversity and shifted community composition. Such changes were not observed in passaged compost. Some bacterial taxa were enriched or depleted by passaging, e.g., Pseudomonas spp. (hypothesized as a stimulus to A. bisporus fructification) were depleted in passaged compost and casing. Overall, this work presents an experimental approach to manipulate compost and casing microbial communities with the potential to be combined with currently employed cultivation strategies toward a more sustainable mushroom cultivation system.
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U2 - 10.1128/spectrum.01978-23
DO - 10.1128/spectrum.01978-23
M3 - Article
C2 - 37831469
AN - SCOPUS:85180003457
SN - 2165-0497
VL - 11
JO - Microbiology Spectrum
JF - Microbiology Spectrum
IS - 6
ER -