TY - JOUR
T1 - Manipulation of flower structure in transgenic tobacco
AU - Mandel, M. Alejandra
AU - Bowman, John L.
AU - Kempin, Sherry A.
AU - Ma, Hong
AU - Meyerowitz, Elliot M.
AU - Yanofsky, Martin F.
N1 - Funding Information:
analyses, and Harry Klee for providing the B. napus cDNA library. We also thank Desmond Bradley, Rosemary Carpenter, and Enrico Coen for allowing us to cite unpublished data. This work was supported by a grant from the National Science Foundation (DC59018749), funds from the Powell Foundation, and a David and Lucille Packard Fellowship in Science and Engineering to M. F. Y. and by a National Institutes of Health (NIH) grant (GM45897) to E. M. M. J. L. B. was supported in part by NIH training grant 5T32GM07818.
PY - 1992/10/2
Y1 - 1992/10/2
N2 - Genetic studies suggest that three homeotic functions, designated A, B, and C, act alone and together to specify the fate of floral organ primordia in distantly related dicotyledonous plant species. To test the genetic model, we have generated transgenic tobacco plants that ectopically express the AGAMOUS gene from Brassica napus, which is necessary for the C function. Flowers on the resulting plants showed homeotic transformations of sepals into carpels and petals into stamens. These phenotypes are consistent with predictions from the genetic model, show that expression of AGAMOUS is sufficient to provide ectopic C function, and demonstrate that the structure of flowers can be manipulated in a predictable manner by altering the expression of a single regulatory gene. Furthermore, the generation of the predicted transformations by ectopic expression of the Brassica gene in transgenic tobacco indicates that gene functions are interchangable between phylogenetically distant species.
AB - Genetic studies suggest that three homeotic functions, designated A, B, and C, act alone and together to specify the fate of floral organ primordia in distantly related dicotyledonous plant species. To test the genetic model, we have generated transgenic tobacco plants that ectopically express the AGAMOUS gene from Brassica napus, which is necessary for the C function. Flowers on the resulting plants showed homeotic transformations of sepals into carpels and petals into stamens. These phenotypes are consistent with predictions from the genetic model, show that expression of AGAMOUS is sufficient to provide ectopic C function, and demonstrate that the structure of flowers can be manipulated in a predictable manner by altering the expression of a single regulatory gene. Furthermore, the generation of the predicted transformations by ectopic expression of the Brassica gene in transgenic tobacco indicates that gene functions are interchangable between phylogenetically distant species.
UR - https://www.scopus.com/pages/publications/0026775057
UR - https://www.scopus.com/pages/publications/0026775057#tab=citedBy
U2 - 10.1016/0092-8674(92)90272-E
DO - 10.1016/0092-8674(92)90272-E
M3 - Article
C2 - 1356631
AN - SCOPUS:0026775057
SN - 0092-8674
VL - 71
SP - 133
EP - 143
JO - Cell
JF - Cell
IS - 1
ER -