Mapping and confi rmation of a new allele at rpp1 from soybean pi 594538a conferring rb lesion-type resistance to soybean rust

Soybean rust, caused by Phakopsora pachyrhizi (H. Sydow & Sydow), is a destructive soybean [Glycine max (L.) Merr.] disease and identifi - cation of new resistance genes is essential for effective rust management. Our research objectives were to map and confi rm the location of resistance gene(s) in PI 594538A using a population of 98 F3:4 lines from a cross between PI 594538A (reddish-brown [RB] lesions) and the susceptible cultivar Loda (tan [TAN] lesions). The lines were inoculated with the P. pachyrhizi isolate ZM01-1 from Zimbabwe. The RB resistance in PI 594538A mapped on linkage group G as a single dominant gene. This gene is likely an allele of Rpp1 or a new closely linked gene because it mapped within 1 cM of Rpp1 and ZM01-1 produced RB lesions on PI 594538A and TAN lesions on PI 200492, the original source of Rpp1. The mapping of the new Rpp1 allele, named Rpp1-b, was confi rmed in a second population.