Mass Spectrometry-Based Vitreous Proteomics: Validated Methods and Analysis Pipeline

Sarah Weber, Nick Carruthers, Chris Gates, Yuanjun Zhao, Jeffrey Sundstrom

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Retinal diseases like diabetic retinopathy and age-related macular degeneration affect millions of individuals worldwide and often lead to vision loss. Vitreous fluid abuts the retina, is accessible for sampling, and contains many proteins related to retinal disease. Therefore, analysis of vitreous is an important tool for studying retinal disease. Because it is rich in proteins and extracellular vesicles, mass spectrometry-based proteomics is an excellent method for vitreous analysis. Here, we discuss important variables to consider when performing vitreous proteomics via mass spectrometry.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages157-167
Number of pages11
DOIs
StatePublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2678
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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