Massively Parallel Sequencing of the Mitogenome from Human Hair Shafts in Forensic Investigations

Jade T. Korber, Lauren C. Canale, Mitchell M. Holland

Research output: Contribution to journalArticlepeer-review


This article highlights methods used to perform DNA extraction, mitochondrial DNA quantification, multiplex PCR amplification, amplicon-based massively parallel sequencing, and data analysis of the mitochondrial genome (mitogenome) from human hair shafts. The focus is on applications to forensic casework, but this set of protocols can be used for any purpose involving small cuttings (as small as 1 to 5 mm) of human hair shafts up to 40 years from the time of collection.

Original languageEnglish (US)
Article numbere865
JournalCurrent Protocols
Issue number8
StatePublished - Aug 2023

All Science Journal Classification (ASJC) codes

  • General Neuroscience
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Health Informatics
  • Medical Laboratory Technology

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