Maturation specific sensitivity of osteocytes to calcium and phosphate ion pair‐induced apoptosis

Matthew Debo, Ray Saunders, Angela Genoese, Christopher Adams

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Our previous work demonstrated the sensitivity of bone cells to microenvironmental apoptogens. As the lifespan of an osteocyte can extend into decades, osteocytic terminal differentiation must necessarily remove such sensitivity. Therefore, the goal of this study is to probe the relationship between osteocyte differentiation state and sensitivity to apoptosis. Murine Long bone‐derived osteocytes (MLO) A5 and Y4 cell lines, representative of early and late stage osteocytes, respectively, were seeded onto well plates, allowed to grow for 2, 7 or 14 days and subsequently exposed to increased levels of the calcium and phosphate ion pair for 24 h. Following exposure, MTT assays were performed to quantify cell death. Apoptosis was confirmed through caspase‐3 and TUNEL staining. Osteocytic phenotypes were confirmed through alkaline phosphatase staining. Results indicated that, when treated with increased levels of the calcium and phosphate ion pair, the MLO A5 cell line, representative of early osteocytes, exhibited greater sensitivity to apoptosis than the MLO Y4 cell line, representative of late osteocytes. These data suggest that sensitivity to calcium and phosphate induced apoptosis is a function of maturation level. This work was supported by a grant from the Center for Chronic Disorders of Aging at the Philadelphia College of Osteopathic Medicine.
Original languageEnglish (US)
Title of host publicationThe FASEB Journal
PublisherFederation of American Societies for Experimental Biology
ISBN (Electronic)1530-6860
ISBN (Print)0892-6638
StatePublished - Apr 2012
EventExperimental Biology 2012 - San Diego, United States
Duration: Apr 21 2012Apr 25 2012


ConferenceExperimental Biology 2012
Country/TerritoryUnited States
CitySan Diego
Internet address


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