Chick, turkey, and duck serum samples were analyzed for free amino acid content by a recently developed reverse-phase high-performance liquid chromatography (HPLC) method and by conventional ion-exchange chromatography using an amino acid analyzer. The former method employed precolumn derivatization with o-phthalaldehyde-ethanethiol and fluorescence detection. With the HPLC procedure, 17 amino acids were separated and quantitated with a total run time of 70 min (column regeneration time included), while the ion-exchange method required a run time of approximately 270 min. In the former procedure, glycine and threonine were not resolved and cyst(e)ine and proline were not detected. Within a species, the serum amino acid concentrations obtained by the two methods were very similar with the exception of asparagine, lysine, and tryptophan. Levels of the first two were consistently higher when analyzed by ion-exchange chromatography, while tryptophan values were consistently greater when analyzed by HPLC. These results suggest that free amino acid analyses of avian serum can be both accurately and reproducibly achieved by HPLC.
All Science Journal Classification (ASJC) codes
- Agricultural and Biological Sciences(all)