Measuring the fluorescent quantum efficiency of indocyanine green encapsulated in nanocomposite particulates

T. J. Russin, E. Ï Altmoǧlu, J. H. Adair, P. C. Eklund

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


We present results of a fluorescent quantum efficiency (ΦF) study on the encapsulation of the near-infrared dye indocyanine green (ICG) in bioresorbable calcium phosphate nanoparticles (CPNPs). The ΦF (described as the ratio of photons emitted to photons absorbed) provides a quantitative means of describing the fluorescence of an arbitrary molecule. However, standard quantum efficiency measurement techniques provide only the ΦF of the smallest fluorescing unit-in the case of a nanoparticle suspension, the nanoparticle itself. This presents a problem in accurately describing the ΦF of fluorophores embedded in an inorganic nanoparticle. Combining the incidence of scattering with an evaluation of the differences in local electric field and photochemical environment, we have developed a method to determine the ΦF of the constituent fluorescent molecules embedded in such a nanoparticle, which provides a more meaningful comparison with the unencapsulated fluorophore. While applicable to generic systems, we present results obtained by our method for the ICG-CPNP in a phosphate buffered 0.15 M saline solution (PBS, pH 7.4)-specifically, ΦF,free dye = 0.027 ± 0.001, ΦF,particle = 0.053 ± 0.003, and for the individual encapsulated molecules, ΦF, molecule = 0.066 ± 0.004. The method developed also provides insight into the influences of encapsulation and key parameters to engineer resonant enhancement effects from the emission of the encapsulated fluorophores corresponding to an eigenmode of the embedding particle for tailored optical properties. (Some figures in this article are in colour only in the electronic version)

Original languageEnglish (US)
Article number334217
JournalJournal of Physics Condensed Matter
Issue number33
StatePublished - Aug 4 2010

All Science Journal Classification (ASJC) codes

  • General Materials Science
  • Condensed Matter Physics


Dive into the research topics of 'Measuring the fluorescent quantum efficiency of indocyanine green encapsulated in nanocomposite particulates'. Together they form a unique fingerprint.

Cite this