@inbook{a32b6ecaa0cb4c33a173dcd4152f68cc,
title = "Mechanism-Based Strategies for Structural Characterization of Radical SAM Reaction Intermediates",
abstract = "X-ray crystallographic characterization of enzymes at different stages in their reaction cycles can provide unique insight into the reaction pathway, the number and type of intermediates formed, and their structural context. The known mechanistic diversity in the radical S-adenosylmethionine (SAM) superfamily of enzymes makes it an appealing target for such studies as more than 100,000 sequences have been identified to date with wide-ranging reactivities that share a pattern of complex radical-mediated chemistry. Here, we review selected examples of radical SAM enzyme crystal structures representative of reactant, product, and intermediate state complexes with a particular emphasis on the strategies employed to capture these states. Broader application of structural characterization techniques to analyze mechanism and substrate specificity is certain to play an important role as more members of this family become tractable for biochemical study.",
author = "Davis, {Katherine M.} and Boal, {Amie K.}",
note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",
year = "2017",
doi = "10.1016/bs.mie.2017.07.008",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "331--359",
booktitle = "Methods in Enzymology",
address = "United States",
}