TY - JOUR
T1 - Mechanism of transcriptional activation by Sp1
T2 - Evidence for coactivators
AU - Pugh, B. Franklin
AU - Tjian, Robert
N1 - Funding Information:
Kane, S. McKnight, and K. Yamamoto for critical reading of the manuscript. B. F. P is a fellow of the Leukemia Society of America. This work was partly funded by a grant from the National Institutes of Health to R. T.
PY - 1990/6/29
Y1 - 1990/6/29
N2 - In reconstituted reactions, Sp1 stimulates transcription at TATA-containing promoters in the presence of semipurified TFIID fractions from either human or Drosophila cells, but is unable to do so when these fractions are replaced by purified, cloned Drosophila or yeast TFIID. Our findings with Sp1 and CTF suggest that partially purified TFIID fractions from human and Drosophila cells contain coactivators that are dispensable for basal transcription but are required as molecular adaptors between trans-activators and the general transcription initiation machinery. Experiments using cloned TFIID proteins suggest that these coactivators function through the amino-terminal portion of TFIID and that coactivator-TFIID interactions are species specific. At promoters lacking a TATA box, an additional activity distinct from coactivators is required for Sp1 activation of transcription.
AB - In reconstituted reactions, Sp1 stimulates transcription at TATA-containing promoters in the presence of semipurified TFIID fractions from either human or Drosophila cells, but is unable to do so when these fractions are replaced by purified, cloned Drosophila or yeast TFIID. Our findings with Sp1 and CTF suggest that partially purified TFIID fractions from human and Drosophila cells contain coactivators that are dispensable for basal transcription but are required as molecular adaptors between trans-activators and the general transcription initiation machinery. Experiments using cloned TFIID proteins suggest that these coactivators function through the amino-terminal portion of TFIID and that coactivator-TFIID interactions are species specific. At promoters lacking a TATA box, an additional activity distinct from coactivators is required for Sp1 activation of transcription.
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U2 - 10.1016/0092-8674(90)90683-6
DO - 10.1016/0092-8674(90)90683-6
M3 - Article
C2 - 2194667
AN - SCOPUS:0025351398
SN - 0092-8674
VL - 61
SP - 1187
EP - 1197
JO - Cell
JF - Cell
IS - 7
ER -