Abstract
Ultracentrifugation of very dilute suspensions of herpes simplex virus directly onto monolayer cells grown in centrifuge tubes was studied. Enhanced infectivity by ultracentrifugation was similar at 4°C and at 35 to 37°C. The high infectivity levels of cultures centrifuged at 4°C were further examined by infectious center assays. At 4°C, the numbers of infectious centers in control (noncentrifuged) cultures were almost 100-fold fewer than in control cultures at 37°C. However, the numbers of infectious centers in cultures ultracentrifuged at 4°C were similar to those ultracentrifuged at 37°C. The great difference in the numbers of infectious centers between 4 and 37°C control cultures, in contrast to the similarity between 4 and 37°C ultracentrifuged cultures, indicated that ultracentrifugation at 4°C enhanced infectivity possibly by facilitation of herpes simplex virus penetration into monolayer cells.
Original language | English (US) |
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Pages (from-to) | 193-197 |
Number of pages | 5 |
Journal | Infection and Immunity |
Volume | 30 |
Issue number | 1 |
DOIs | |
State | Published - 1980 |
All Science Journal Classification (ASJC) codes
- Parasitology
- Microbiology
- Immunology
- Infectious Diseases