TY - CHAP
T1 - Mechanistic analysis of the Hepatitis Delta Virus (HDV) ribozyme
T2 - Methods for RNA preparation, structure mapping, solvent isotope effects, and co-transcriptional cleavage
AU - Chadalavada, Durga M.
AU - Cerrone-Szakal, Andrea L.
AU - Wilcox, Jennifer L.
AU - Siegfried, Nathan A.
AU - Bevilacqua, Philip C.
PY - 2012
Y1 - 2012
N2 - Small ribozymes such as the hairpin, hammerhead, VS, glm S, and hepatitis delta virus (HDV) are self-cleaving RNAs that are typically characterized by kinetics and structural methods. Working with these RNAs requires attention to numerous experimental details. In this chapter we focus on four different experimental aspects of ribozyme studies: preparing the RNA, mapping its structure with reverse transcription and end-labeled techniques, solvent isotope experiments, and co-transcriptional cleavage assays. Although the focus of these methods is the HDV ribozyme, the methods should be applicable to other ribozymes.
AB - Small ribozymes such as the hairpin, hammerhead, VS, glm S, and hepatitis delta virus (HDV) are self-cleaving RNAs that are typically characterized by kinetics and structural methods. Working with these RNAs requires attention to numerous experimental details. In this chapter we focus on four different experimental aspects of ribozyme studies: preparing the RNA, mapping its structure with reverse transcription and end-labeled techniques, solvent isotope experiments, and co-transcriptional cleavage assays. Although the focus of these methods is the HDV ribozyme, the methods should be applicable to other ribozymes.
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U2 - 10.1007/978-1-61779-545-9_3
DO - 10.1007/978-1-61779-545-9_3
M3 - Chapter
C2 - 22315061
AN - SCOPUS:84858236151
SN - 9781617795442
T3 - Methods in Molecular Biology
SP - 21
EP - 40
BT - Ribozymes
A2 - Hartig, Jorg
ER -