Melting curve SNP (McSNP) genotyping: A useful approach for diallelic genotyping in forensic science

Jian Ye; Esteban J. Parra; Donna M. Sosnoski; Kevin Hiester; P. A. Underhill; Mark D. Shriver

doi:10.1520/jfs2001183

Melting curve SNP (McSNP) genotyping: A useful approach for diallelic genotyping in forensic science

Jian Ye, Esteban J. Parra, Donna M. Sosnoski, Kevin Hiester, P. A. Underhill, Mark D. Shriver

Anthropology

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45 Scopus citations

Abstract

The increasing availability of Single Nucleotide Polymorphisms (SNPs) and Deletion/Insertion Polymorphisms (DIPs), as well as the outstanding progress in SNP genotyping technologies, will impact forensics profoundly. We have developed a new method for genotyping SNPs and DIPs, which is based on the determination of melting curve profiles of amplified DNA in solution. We have termed this method Melting curve SNP (McSNP) genotyping. Melting curve profiles are composites of the particular melting temperatures (T_m) of the individual fragments that comprise the DNA sample. Simple mixtures of DNA can be resolved in a very robust and efficient fashion, since the samples can be scored in the plates in which they were amplified with no or very few post-PCR manipulations. As such, McSNP is one of the least expensive genotyping methods available and can and should be useful in forensic science.

Original language	English (US)
Pages (from-to)	593-600
Number of pages	8
Journal	Journal of Forensic Sciences
Volume	47
Issue number	3
DOIs	https://doi.org/10.1520/jfs2001183
State	Published - 2002

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Genetics

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abstract = "The increasing availability of Single Nucleotide Polymorphisms (SNPs) and Deletion/Insertion Polymorphisms (DIPs), as well as the outstanding progress in SNP genotyping technologies, will impact forensics profoundly. We have developed a new method for genotyping SNPs and DIPs, which is based on the determination of melting curve profiles of amplified DNA in solution. We have termed this method Melting curve SNP (McSNP) genotyping. Melting curve profiles are composites of the particular melting temperatures (Tm) of the individual fragments that comprise the DNA sample. Simple mixtures of DNA can be resolved in a very robust and efficient fashion, since the samples can be scored in the plates in which they were amplified with no or very few post-PCR manipulations. As such, McSNP is one of the least expensive genotyping methods available and can and should be useful in forensic science.",

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T1 - Melting curve SNP (McSNP) genotyping

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AU - Ye, Jian

AU - Parra, Esteban J.

AU - Sosnoski, Donna M.

AU - Hiester, Kevin

AU - Underhill, P. A.

AU - Shriver, Mark D.

PY - 2002

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AB - The increasing availability of Single Nucleotide Polymorphisms (SNPs) and Deletion/Insertion Polymorphisms (DIPs), as well as the outstanding progress in SNP genotyping technologies, will impact forensics profoundly. We have developed a new method for genotyping SNPs and DIPs, which is based on the determination of melting curve profiles of amplified DNA in solution. We have termed this method Melting curve SNP (McSNP) genotyping. Melting curve profiles are composites of the particular melting temperatures (Tm) of the individual fragments that comprise the DNA sample. Simple mixtures of DNA can be resolved in a very robust and efficient fashion, since the samples can be scored in the plates in which they were amplified with no or very few post-PCR manipulations. As such, McSNP is one of the least expensive genotyping methods available and can and should be useful in forensic science.

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Melting curve SNP (McSNP) genotyping: A useful approach for diallelic genotyping in forensic science

Abstract

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