Membrane association and conformational change of palmitoylated Goα

Shengyu Yang; Ling Zhang; Youguo Huang

doi:10.1016/S0014-5793(01)02493-0

Membrane association and conformational change of palmitoylated G_oα

Shengyu Yang, Ling Zhang, Youguo Huang

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Bovine brain G_oα was specifically palmitoylated in vitro. The apparent dissociation constant for depalmitoylated G_oα (dG_oα) was 0.273 μM, while that for palmitoylated G_oα (pG_oα) was 5.77 nM. The dissociation rate constant (K₂₁) and dissociation half-life for dG_oα were 8.4 × 10^-4 min and 825 min respectively, while no significant dissociation of pG_oα was detected. The limiting membrane insertion pressures for pG_oα and dG_oα were 44.4 mN/m and 41.3 mN/m respectively. These data suggested that palmitoylation facilitated the membrane association of G_oα. Conformational changes of dG_oα and pG_oα detected by monitoring fluorescence spectra and fluorescence quenching were significantly different after they were associated with the membrane. It was suggested that conformational changes of G_oα upon membrane association might be related to regulation of G_oα signaling by palmitoylation.

Original language	English (US)
Pages (from-to)	76-81
Number of pages	6
Journal	FEBS Letters
Volume	498
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(01)02493-0
State	Published - Jun 1 2001

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(01)02493-0

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title = "Membrane association and conformational change of palmitoylated Goα",

abstract = "Bovine brain Goα was specifically palmitoylated in vitro. The apparent dissociation constant for depalmitoylated Goα (dGoα) was 0.273 μM, while that for palmitoylated Goα (pGoα) was 5.77 nM. The dissociation rate constant (K21) and dissociation half-life for dGoα were 8.4 × 10-4 min and 825 min respectively, while no significant dissociation of pGoα was detected. The limiting membrane insertion pressures for pGoα and dGoα were 44.4 mN/m and 41.3 mN/m respectively. These data suggested that palmitoylation facilitated the membrane association of Goα. Conformational changes of dGoα and pGoα detected by monitoring fluorescence spectra and fluorescence quenching were significantly different after they were associated with the membrane. It was suggested that conformational changes of Goα upon membrane association might be related to regulation of Goα signaling by palmitoylation.",

author = "Shengyu Yang and Ling Zhang and Youguo Huang",

note = "Funding Information: This work was generously supported by Project 39730130 from the National Natural Science Foundation of China and Key Project KJ951-B1-609 from the Chinese Academy of Sciences. The authors are grateful to Prof. Fuyu Yang for his helpful discussions, to Dr. Teresa Jones for valuable comments, to Dr. Jiachang Yue for HB, and to Dr. Xuehai Han for the membrane-balance experiment.",

year = "2001",

month = jun,

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doi = "10.1016/S0014-5793(01)02493-0",

language = "English (US)",

volume = "498",

pages = "76--81",

journal = "FEBS Letters",

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publisher = "John Wiley and Sons Inc.",

number = "1",

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T1 - Membrane association and conformational change of palmitoylated Goα

AU - Yang, Shengyu

AU - Zhang, Ling

AU - Huang, Youguo

N1 - Funding Information: This work was generously supported by Project 39730130 from the National Natural Science Foundation of China and Key Project KJ951-B1-609 from the Chinese Academy of Sciences. The authors are grateful to Prof. Fuyu Yang for his helpful discussions, to Dr. Teresa Jones for valuable comments, to Dr. Jiachang Yue for HB, and to Dr. Xuehai Han for the membrane-balance experiment.

PY - 2001/6/1

Y1 - 2001/6/1

N2 - Bovine brain Goα was specifically palmitoylated in vitro. The apparent dissociation constant for depalmitoylated Goα (dGoα) was 0.273 μM, while that for palmitoylated Goα (pGoα) was 5.77 nM. The dissociation rate constant (K21) and dissociation half-life for dGoα were 8.4 × 10-4 min and 825 min respectively, while no significant dissociation of pGoα was detected. The limiting membrane insertion pressures for pGoα and dGoα were 44.4 mN/m and 41.3 mN/m respectively. These data suggested that palmitoylation facilitated the membrane association of Goα. Conformational changes of dGoα and pGoα detected by monitoring fluorescence spectra and fluorescence quenching were significantly different after they were associated with the membrane. It was suggested that conformational changes of Goα upon membrane association might be related to regulation of Goα signaling by palmitoylation.

AB - Bovine brain Goα was specifically palmitoylated in vitro. The apparent dissociation constant for depalmitoylated Goα (dGoα) was 0.273 μM, while that for palmitoylated Goα (pGoα) was 5.77 nM. The dissociation rate constant (K21) and dissociation half-life for dGoα were 8.4 × 10-4 min and 825 min respectively, while no significant dissociation of pGoα was detected. The limiting membrane insertion pressures for pGoα and dGoα were 44.4 mN/m and 41.3 mN/m respectively. These data suggested that palmitoylation facilitated the membrane association of Goα. Conformational changes of dGoα and pGoα detected by monitoring fluorescence spectra and fluorescence quenching were significantly different after they were associated with the membrane. It was suggested that conformational changes of Goα upon membrane association might be related to regulation of Goα signaling by palmitoylation.

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Membrane association and conformational change of palmitoylated G_oα

Abstract

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