TY - JOUR
T1 - Metabolic flux analysis reaching genome wide coverage
T2 - lessons learned and future perspectives
AU - Hendry, John I.
AU - Dinh, Hoang V.
AU - Foster, Charles
AU - Gopalakrishnan, Saratram
AU - Wang, Lin
AU - Maranas, Costas D.
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/12
Y1 - 2020/12
N2 - 13C-MFA is currently the only technique capable of elucidating intracellular metabolic fluxes. Generally, in 13C-MFA studies the reactions that can carry flux are mostly pre-specified by only considering canonical pathways and ignoring alternate ones. This may bias flux elucidation and cause labeling data to erroneously confirm implied assumptions. By expanding the scope of the metabolic mapping models to match known genome-scale metabolism such estimation biases can be eliminated. However, this model expansion to genome-scale requires the construction of expanded atom mapping models, more efficient flux estimation algorithms, and formal estimation of confidence levels. Even though significant progress has been made in this direction, a number of challenges remain before widespread adoption by the community.
AB - 13C-MFA is currently the only technique capable of elucidating intracellular metabolic fluxes. Generally, in 13C-MFA studies the reactions that can carry flux are mostly pre-specified by only considering canonical pathways and ignoring alternate ones. This may bias flux elucidation and cause labeling data to erroneously confirm implied assumptions. By expanding the scope of the metabolic mapping models to match known genome-scale metabolism such estimation biases can be eliminated. However, this model expansion to genome-scale requires the construction of expanded atom mapping models, more efficient flux estimation algorithms, and formal estimation of confidence levels. Even though significant progress has been made in this direction, a number of challenges remain before widespread adoption by the community.
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U2 - 10.1016/j.coche.2020.05.008
DO - 10.1016/j.coche.2020.05.008
M3 - Review article
AN - SCOPUS:85087376771
SN - 2211-3398
VL - 30
SP - 17
EP - 25
JO - Current Opinion in Chemical Engineering
JF - Current Opinion in Chemical Engineering
ER -