TY - JOUR
T1 - Metal reduction at low pH by a Desulfosporosinusspecies
T2 - Implications for the biological treatment of acidic mine drainage
AU - Senko, John M.
AU - Zhang, Gengxin
AU - McDonough, Jeffrey T.
AU - Bruns, Mary Ann
AU - Burgos, William D.
N1 - Funding Information:
We thank Pam Milavec and Jon Smoyer from the Pennsylvania Department of Environmental Protection for directing us to the Gum Boot system. We thank David Watson from the Oak Ridge National Laboratory for providing groundwater samples. This work was supported by the Pennsylvania Department of Environmental Protection, Bureau of Abandoned Mine Reclamation, the Pennsylvania Water Resources Research Center at the Pennsylvania State University, the Environmental Remediation Science Program (ERSP), Office of Biological and Environmental Research (OBER), Office of Energy Research, US Department of Energy (DOE) grant no. DE-FG02-04ER63914, and the National Science Foundation and US DOE OBER under grant no. CHE-0431328.
PY - 2009
Y1 - 2009
N2 - We isolated an acid-tolerant sulfate-reducing bacterium, GBSRB4.2, from coal mine-derived acidic mine drainage (AMD)-derived sediments. Sequence analysis of partial 16S rRNA gene of GBSRB4.2 revealed that it was affiliated with the genus Desulfosporosinus. GBSRB4.2 reduced sulfate, Fe(III) (hydr)oxide, Mn(IV) oxide, and U(VI) in acidic solutions (pH 4.2). Sulfate, Fe(III), and Mn(IV) but not U(VI) bioreduction led to an increase in the pH of acidic solutions and concurrent hydrolysis and precipitation of dissolved Al3+. Reduction of Fe(III), Mn(IV), and U(VI) in sulfate-free solutions revealed that these metals are enzymatically reduced by GBSRB4.2. GBSRB4.2 reduced U(VI) in groundwater from a radionuclide-contaminated aquifer more rapidly at pH 4.4 than at pH 7.1, possibly due to the formation of poorly bioreducible Ca-U(VI)-CO3 complexes in the pH 7.1 groundwater.
AB - We isolated an acid-tolerant sulfate-reducing bacterium, GBSRB4.2, from coal mine-derived acidic mine drainage (AMD)-derived sediments. Sequence analysis of partial 16S rRNA gene of GBSRB4.2 revealed that it was affiliated with the genus Desulfosporosinus. GBSRB4.2 reduced sulfate, Fe(III) (hydr)oxide, Mn(IV) oxide, and U(VI) in acidic solutions (pH 4.2). Sulfate, Fe(III), and Mn(IV) but not U(VI) bioreduction led to an increase in the pH of acidic solutions and concurrent hydrolysis and precipitation of dissolved Al3+. Reduction of Fe(III), Mn(IV), and U(VI) in sulfate-free solutions revealed that these metals are enzymatically reduced by GBSRB4.2. GBSRB4.2 reduced U(VI) in groundwater from a radionuclide-contaminated aquifer more rapidly at pH 4.4 than at pH 7.1, possibly due to the formation of poorly bioreducible Ca-U(VI)-CO3 complexes in the pH 7.1 groundwater.
UR - http://www.scopus.com/inward/record.url?scp=61349202855&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=61349202855&partnerID=8YFLogxK
U2 - 10.1080/01490450802660193
DO - 10.1080/01490450802660193
M3 - Article
AN - SCOPUS:61349202855
SN - 0149-0451
VL - 26
SP - 71
EP - 82
JO - Geomicrobiology Journal
JF - Geomicrobiology Journal
IS - 2
ER -