Methods for exosome isolation and characterization

Mi Zhou, Sarah R. Weber, Yuanjun Zhao, Han Chen, Jeffrey M. Sundstrom

Research output: Chapter in Book/Report/Conference proceedingChapter

43 Scopus citations

Abstract

Extracellular vesicles are released from cells under both physiological and pathophysiological conditions. Exosomes are a class of extracellular vesicles that originate from intraluminal membranes, and their diameters vary from 30 to 150nm. Like hormones and cytokines, exosomes play a critical role in cell-cell communication. Proper methods for exosome isolation and characterization facilitate accurate downstream analyses. This review summarizes both conventional and novel techniques for exosome isolation and characterization as well as their advantages and limitations. Novel methods for fractionating exosome subsets, defining exosomal cargo, tracking exosomes in vivo, and determining whether a protein is associated with the inside or outside of exosomes are also included in this review.

Original languageEnglish (US)
Title of host publicationExosomes
Subtitle of host publicationA Clinical Compendium
PublisherElsevier
Pages23-38
Number of pages16
ISBN (Electronic)9780128160534
ISBN (Print)9780128160541
DOIs
StatePublished - Jan 1 2019

All Science Journal Classification (ASJC) codes

  • General Biochemistry, Genetics and Molecular Biology

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