TY - JOUR
T1 - Micro sensors
T2 - Linking real-time oscillatory shear stress with vascular inflammatory responses
AU - Hsiai, Tzung K.
AU - Cho, Sung K.
AU - Wong, Pak K.
AU - Ing, Michael H.
AU - Salazar, Adler
AU - Hama, Susan
AU - Navab, Mohamad
AU - Demer, Linda L.
AU - Ho, Chih Ming
N1 - Funding Information:
T.K.H. is supported by grants AHA BGIA (0265166U), NIH Career Development Award (K08 HL068689-01A1), and National Heart Foundation Research Grant (AHAF: H2003-028). This work was also supported by the DARPA-Bioflip Project and by the NIH NRSA #HL07895.
PY - 2004/2
Y1 - 2004/2
N2 - The important interplay between blood circulation and vascular cell behavior warrants the development of highly sensitive but small sensing systems. The emerging micro electro mechanical systems (MEMS) technology, thus, provides the high spatiotemporal resolution to link biomechanical forces on the microscale with large-scale physiology. We fabricated MEMS sensors, comparable to the endothelial cells (ECs) in size, to link real-time shear stress with monocyte/EC interactions in an oscillatory flow environment, simulating the moving and unsteady separation point at arterial bifurcations. In response to oscillatory shear stress (τ) at ± 2.6 dyn/cm 2, time-averaged shear stress (τ ave) = 0 at 0.5 Hz, individual monocytes displayed unique to-and-fro trajectories, undergoing rolling, binding, and dissociation with other monocyte, followed by solid adhesion on EC. Incorporating with cell-tracking velocimetry, we visualized that these real-time events occurred over a dynamic range of oscillating shear stress between ±2.6 dyn/cm 2 and Reynolds number between 0 and 22.2 in the presence of activated adhesion molecule and chemokine mRNA expression.
AB - The important interplay between blood circulation and vascular cell behavior warrants the development of highly sensitive but small sensing systems. The emerging micro electro mechanical systems (MEMS) technology, thus, provides the high spatiotemporal resolution to link biomechanical forces on the microscale with large-scale physiology. We fabricated MEMS sensors, comparable to the endothelial cells (ECs) in size, to link real-time shear stress with monocyte/EC interactions in an oscillatory flow environment, simulating the moving and unsteady separation point at arterial bifurcations. In response to oscillatory shear stress (τ) at ± 2.6 dyn/cm 2, time-averaged shear stress (τ ave) = 0 at 0.5 Hz, individual monocytes displayed unique to-and-fro trajectories, undergoing rolling, binding, and dissociation with other monocyte, followed by solid adhesion on EC. Incorporating with cell-tracking velocimetry, we visualized that these real-time events occurred over a dynamic range of oscillating shear stress between ±2.6 dyn/cm 2 and Reynolds number between 0 and 22.2 in the presence of activated adhesion molecule and chemokine mRNA expression.
UR - http://www.scopus.com/inward/record.url?scp=2942558759&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2942558759&partnerID=8YFLogxK
U2 - 10.1023/B:ABME.0000012739.88554.01
DO - 10.1023/B:ABME.0000012739.88554.01
M3 - Article
C2 - 15008367
AN - SCOPUS:2942558759
SN - 0090-6964
VL - 32
SP - 189
EP - 201
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
IS - 2
ER -