TY - JOUR
T1 - Microbial ATP bioluminescence as a means to detect contamination on artificially contaminated beef carcass tissue
AU - Siragusa, Gregory R.
AU - Cutter, Catherine N.
PY - 1995/7
Y1 - 1995/7
N2 - The use of microbial ATP bioluminescence was evaluated as a means to rapidly detect gross microbial contamination from feces on bovine-carcass surface tissue (BCT). Microbial ATP was selectively distinguished from nonmicrobial ATP by the assay procedure used. Regression analyses of microbial ATP and viable count scatterplots showed lean and adipose BCT artificially contaminated with bovine feces had the same regression line parameters (P < 0.05), and therefore, the microbial ATP responses were similar for both tissue types. Correlation coefficients (r) of these regression lines were >0.90 for both tissue types. Results indicated that swab samples can be held at 5°C for up to 6 h without compromising microbial ATP bioluminescence assay results. The microbial ATP bioluminescence assay shows potential for use as a means to rapidly detect fecal contamination on red meat carcasses and to gauge decontamination effectiveness and hence could monitor critical control points in a processing-plant HACCP plan.
AB - The use of microbial ATP bioluminescence was evaluated as a means to rapidly detect gross microbial contamination from feces on bovine-carcass surface tissue (BCT). Microbial ATP was selectively distinguished from nonmicrobial ATP by the assay procedure used. Regression analyses of microbial ATP and viable count scatterplots showed lean and adipose BCT artificially contaminated with bovine feces had the same regression line parameters (P < 0.05), and therefore, the microbial ATP responses were similar for both tissue types. Correlation coefficients (r) of these regression lines were >0.90 for both tissue types. Results indicated that swab samples can be held at 5°C for up to 6 h without compromising microbial ATP bioluminescence assay results. The microbial ATP bioluminescence assay shows potential for use as a means to rapidly detect fecal contamination on red meat carcasses and to gauge decontamination effectiveness and hence could monitor critical control points in a processing-plant HACCP plan.
UR - http://www.scopus.com/inward/record.url?scp=0028890129&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028890129&partnerID=8YFLogxK
U2 - 10.4315/0362-028X-58.7.764
DO - 10.4315/0362-028X-58.7.764
M3 - Article
AN - SCOPUS:0028890129
SN - 0362-028X
VL - 58
SP - 764
EP - 769
JO - Journal of food protection
JF - Journal of food protection
IS - 7
ER -