TY - JOUR
T1 - Microbial ecology of the stratified water column of the Black Sea as revealed by a comprehensive biomarker study
AU - Wakeham, Stuart G.
AU - Amann, Rudi
AU - Freeman, Katherine H.
AU - Hopmans, Ellen C.
AU - Jørgensen, Bo Barker
AU - Putnam, Isabell F.
AU - Schouten, Stefan
AU - Sinninghe Damsté, Jaap S.
AU - Talbot, Helen M.
AU - Woebken, Dagmar
N1 - Funding Information:
We thank the Captain and crew of the R/V Knorr for assistance during all phases of the cruise, and to James Murray and George Luther III as chief scientists. Krisa Arzayus and Martin Vollmer assisted in sampling. The Max Planck Institute for Marine Microbiology (MPIMM), Bremen, provided facilities support for SGW to carry out the CARD-FISH investigation. Gordon Taylor and Xueju Lin at Stony Brook University (SBU) provided the ESP459 data, Marianne Baas at NIOZ conducted the ladderane analyses, and Chris Lernihan ran compound specific isotope analyses at Pennsylvania State University. We thank Mark Teece and an anonymous reviewer for constructive suggestions on improving the manuscript. SGW acknowledges the support of the Hanse-Wissenschaftskolleg/Hanse Institute for Advanced Study for a Fellowship in 2003 allowing him to work at the MPIMM. This research was also supported by the US National Science Foundation Chemical Oceanography Program through grants OCE-0117824 and OCE-0550654 to SGW. We thank the U.K. Science Research Infrastructure Fund (SRIF) from HEFCE for funding the purchase of the ThermoFinnigan LCQ ion trap mass spectrometer at the University of Newcastle.
PY - 2007/12
Y1 - 2007/12
N2 - The stratified water column of the Black Sea is partitioned into oxic, suboxic, and euxinic zones, each characterized by different biogeochemical processes and by distinct microbial communities. In 2003, we collected particulate matter by large volume in situ filtration at the highest resolution to date for lipid biomarker analysis and bacterioplankton for enumeration of major prokaryotic groups. Abundances of several prokaryotic groups were estimated using CARD-FISH probes specific for Bacteria, Archaea (Crenarchaeota and Euryarchaeota), epsilonproteobacteria (mainly sulfide oxidizers) and sulfate reducing bacteria. We also measured a wide range of bacterial and archaeal lipid biomarkers. Depth distributions of diagnostic biomarkers are matched with zonation of microbial processes, including aerobic bacterial oxidation of methane, oxidation of ammonium by bacteria and archaea, metal reduction, and sulfide oxidation at the chemocline, and bacterial sulfate reduction and anaerobic oxidation of methane by archaea in the anoxic zone. Cell densities for archaea and sulfate reducing bacteria are estimated based on water column biomarker concentrations and compared with CARD-FISH results.
AB - The stratified water column of the Black Sea is partitioned into oxic, suboxic, and euxinic zones, each characterized by different biogeochemical processes and by distinct microbial communities. In 2003, we collected particulate matter by large volume in situ filtration at the highest resolution to date for lipid biomarker analysis and bacterioplankton for enumeration of major prokaryotic groups. Abundances of several prokaryotic groups were estimated using CARD-FISH probes specific for Bacteria, Archaea (Crenarchaeota and Euryarchaeota), epsilonproteobacteria (mainly sulfide oxidizers) and sulfate reducing bacteria. We also measured a wide range of bacterial and archaeal lipid biomarkers. Depth distributions of diagnostic biomarkers are matched with zonation of microbial processes, including aerobic bacterial oxidation of methane, oxidation of ammonium by bacteria and archaea, metal reduction, and sulfide oxidation at the chemocline, and bacterial sulfate reduction and anaerobic oxidation of methane by archaea in the anoxic zone. Cell densities for archaea and sulfate reducing bacteria are estimated based on water column biomarker concentrations and compared with CARD-FISH results.
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U2 - 10.1016/j.orggeochem.2007.08.003
DO - 10.1016/j.orggeochem.2007.08.003
M3 - Article
AN - SCOPUS:36148976101
SN - 0146-6380
VL - 38
SP - 2070
EP - 2097
JO - Organic Geochemistry
JF - Organic Geochemistry
IS - 12
ER -