Microfluidic device to perform impedometric detection of Activated Partial Thromboplastin Time of blood

B. Ramaswamy; Yin-Ting Yeh; Siyang Zheng

doi:10.1109/TRANSDUCERS.2011.5969391

Microfluidic device to perform impedometric detection of Activated Partial Thromboplastin Time of blood

B. Ramaswamy, Yin-Ting Yeh, Siyang Zheng

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The Activated Partial Thromboplastin Time (APTT) test is performed on a large scale, in laboratories to monitor the functioning of intrinsic and common pathways of blood coagulation cascade and the concentration of anticoagulants such as Heparin among patients. A PDMS based microfluidic device has been fabricated and tested to perform APTT as a point-of-care device using whole blood samples by detecting the change in electrical impedance of blood during coagulation. The two chambered PDMS device integrated with microelectrodes is pre-coated with APTT reagent and heparin respectively for better repeatability. The results indicate a repeatable pattern of change in whole blood impedance during coagulation and confirm the theoretical explanation by Hartert [1]. The devices were also tested for their sensitivity to different concentrations of heparin and found to exhibit a favorable increase in coagulation time for increasing heparin concentration.

Original language	English (US)
Title of host publication	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Pages	222-225
Number of pages	4
DOIs	https://doi.org/10.1109/TRANSDUCERS.2011.5969391
State	Published - Sep 1 2011
Event	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing, China Duration: Jun 5 2011 → Jun 9 2011

Other

Other	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Country/Territory	China
City	Beijing
Period	6/5/11 → 6/9/11

All Science Journal Classification (ASJC) codes

Hardware and Architecture
Electrical and Electronic Engineering

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10.1109/TRANSDUCERS.2011.5969391

Cite this

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title = "Microfluidic device to perform impedometric detection of Activated Partial Thromboplastin Time of blood",

abstract = "The Activated Partial Thromboplastin Time (APTT) test is performed on a large scale, in laboratories to monitor the functioning of intrinsic and common pathways of blood coagulation cascade and the concentration of anticoagulants such as Heparin among patients. A PDMS based microfluidic device has been fabricated and tested to perform APTT as a point-of-care device using whole blood samples by detecting the change in electrical impedance of blood during coagulation. The two chambered PDMS device integrated with microelectrodes is pre-coated with APTT reagent and heparin respectively for better repeatability. The results indicate a repeatable pattern of change in whole blood impedance during coagulation and confirm the theoretical explanation by Hartert [1]. The devices were also tested for their sensitivity to different concentrations of heparin and found to exhibit a favorable increase in coagulation time for increasing heparin concentration.",

author = "B. Ramaswamy and Yin-Ting Yeh and Siyang Zheng",

year = "2011",

month = sep,

day = "1",

doi = "10.1109/TRANSDUCERS.2011.5969391",

language = "English (US)",

isbn = "9781457701573",

pages = "222--225",

booktitle = "2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11",

note = "2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 ; Conference date: 05-06-2011 Through 09-06-2011",

}

Ramaswamy, B, Yeh, Y-T & Zheng, S 2011, Microfluidic device to perform impedometric detection of Activated Partial Thromboplastin Time of blood. in 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11., 5969391, pp. 222-225, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, Beijing, China, 6/5/11. https://doi.org/10.1109/TRANSDUCERS.2011.5969391

TY - GEN

T1 - Microfluidic device to perform impedometric detection of Activated Partial Thromboplastin Time of blood

AU - Ramaswamy, B.

AU - Yeh, Yin-Ting

AU - Zheng, Siyang

PY - 2011/9/1

Y1 - 2011/9/1

N2 - The Activated Partial Thromboplastin Time (APTT) test is performed on a large scale, in laboratories to monitor the functioning of intrinsic and common pathways of blood coagulation cascade and the concentration of anticoagulants such as Heparin among patients. A PDMS based microfluidic device has been fabricated and tested to perform APTT as a point-of-care device using whole blood samples by detecting the change in electrical impedance of blood during coagulation. The two chambered PDMS device integrated with microelectrodes is pre-coated with APTT reagent and heparin respectively for better repeatability. The results indicate a repeatable pattern of change in whole blood impedance during coagulation and confirm the theoretical explanation by Hartert [1]. The devices were also tested for their sensitivity to different concentrations of heparin and found to exhibit a favorable increase in coagulation time for increasing heparin concentration.

AB - The Activated Partial Thromboplastin Time (APTT) test is performed on a large scale, in laboratories to monitor the functioning of intrinsic and common pathways of blood coagulation cascade and the concentration of anticoagulants such as Heparin among patients. A PDMS based microfluidic device has been fabricated and tested to perform APTT as a point-of-care device using whole blood samples by detecting the change in electrical impedance of blood during coagulation. The two chambered PDMS device integrated with microelectrodes is pre-coated with APTT reagent and heparin respectively for better repeatability. The results indicate a repeatable pattern of change in whole blood impedance during coagulation and confirm the theoretical explanation by Hartert [1]. The devices were also tested for their sensitivity to different concentrations of heparin and found to exhibit a favorable increase in coagulation time for increasing heparin concentration.

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UR - http://www.scopus.com/inward/citedby.url?scp=80052118305&partnerID=8YFLogxK

U2 - 10.1109/TRANSDUCERS.2011.5969391

DO - 10.1109/TRANSDUCERS.2011.5969391

M3 - Conference contribution

AN - SCOPUS:80052118305

SN - 9781457701573

SP - 222

EP - 225

BT - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11

T2 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11

Y2 - 5 June 2011 through 9 June 2011

ER -

Microfluidic device to perform impedometric detection of Activated Partial Thromboplastin Time of blood

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