TY - JOUR
T1 - Microfluidic inverse phase ELISA via manipulation of magnetic beads
AU - Chen, Hong
AU - Abolmatty, Assem
AU - Faghri, Mohammad
N1 - Funding Information:
Acknowledgments This work is supported by the National Science Foundation grant (NSF-OISE-0530203). We thank Peng Li for his help to fabricate the SU-8 mold and Prof. Constantine N. Anagnos-topoulos for his helpful suggestions on the manuscript.
PY - 2011/3
Y1 - 2011/3
N2 - We report a new technique for conducting immuno-diagnostics on a microfluidic platform. Rather than handling fluid reagents against a stationary solid phase, the platform manipulates analyte-coated magnetic beads through stationary plugs of fluid reagents to detect an antigenic analyte. These isolated but accessible plugs are preencapsulated in a microchannel by capillary force. We call this platform microfluidic inverse phase enzyme-linked immunosorbent assay (μIPELISA). μIPELISA has distinctive advantages in the family of microfluidic immunoassay. In particular, it avoids pumping and valving fluid reagents during assaying, thus leading to a lab-on-a-chip format that is free of instrumentation for fluid actuation and control. We use μIPELISA to detect digoxigenin-labeled DNA segments amplified from E. coli O157:H7 by polymerase chain reaction (PCR), and compare its detection capability with that of microplate ELISA. For 0.259 ng μl-1 of digoxigenin- labeled amplicon, μIPELISA is as responsive as the microplate ELISA. Also, we simultaneously conduct μIPELISA in two parallel microchannels.
AB - We report a new technique for conducting immuno-diagnostics on a microfluidic platform. Rather than handling fluid reagents against a stationary solid phase, the platform manipulates analyte-coated magnetic beads through stationary plugs of fluid reagents to detect an antigenic analyte. These isolated but accessible plugs are preencapsulated in a microchannel by capillary force. We call this platform microfluidic inverse phase enzyme-linked immunosorbent assay (μIPELISA). μIPELISA has distinctive advantages in the family of microfluidic immunoassay. In particular, it avoids pumping and valving fluid reagents during assaying, thus leading to a lab-on-a-chip format that is free of instrumentation for fluid actuation and control. We use μIPELISA to detect digoxigenin-labeled DNA segments amplified from E. coli O157:H7 by polymerase chain reaction (PCR), and compare its detection capability with that of microplate ELISA. For 0.259 ng μl-1 of digoxigenin- labeled amplicon, μIPELISA is as responsive as the microplate ELISA. Also, we simultaneously conduct μIPELISA in two parallel microchannels.
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U2 - 10.1007/s10404-010-0692-2
DO - 10.1007/s10404-010-0692-2
M3 - Article
AN - SCOPUS:79955944830
SN - 1613-4982
VL - 10
SP - 593
EP - 605
JO - Microfluidics and Nanofluidics
JF - Microfluidics and Nanofluidics
IS - 3
ER -