Micropropagation of Hesperaloe parviflora

Arthur M. Richwine; Jimmy L. Tipton; Gary A. Thompson

doi:10.1007/BF02822698

Micropropagation of Hesperaloe parviflora

Arthur M. Richwine, Jimmy L. Tipton, Gary A. Thompson

College of Agricultural Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We successfully micropropagated Hesperaloe parviflora from mature plants. Shoot cultures were directly initiated from mature plants using pedicel bud explants on a modified Murashige and Skoog medium containing Nitsch and Nitseh vitamins and 1 μM zeatin riboside. Axillary shoot multiplication from established cultures was most responsive to changing concentrations of N⁶-benzyladenine (BA) with the greatest production on 6 μM BA. Growing shoots on a medium supplemented with 6 μM BA for 6 wk and then transferring cultures to a 1 μM BA medium for 6 more wk increased the number of transferable shoots, but not significantly. However, our data predicts that the maximum number of transferable shoots produced from a single microshoot would occur on media with 5.4 μM zeatin riboside. Shoots rooted easily in vitro or ex vitro and rooted shoots were easily acclimatized. The methods described in this paper are being used to commercially micropropagate H. parviflora.

Original language	English (US)
Pages (from-to)	262-266
Number of pages	5
Journal	In Vitro Cellular and Developmental Biology - Plant
Volume	32
Issue number	4
DOIs	https://doi.org/10.1007/BF02822698
State	Published - 1996

All Science Journal Classification (ASJC) codes

Biotechnology
Plant Science

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10.1007/BF02822698

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title = "Micropropagation of Hesperaloe parviflora",

abstract = "We successfully micropropagated Hesperaloe parviflora from mature plants. Shoot cultures were directly initiated from mature plants using pedicel bud explants on a modified Murashige and Skoog medium containing Nitsch and Nitseh vitamins and 1 μM zeatin riboside. Axillary shoot multiplication from established cultures was most responsive to changing concentrations of N6-benzyladenine (BA) with the greatest production on 6 μM BA. Growing shoots on a medium supplemented with 6 μM BA for 6 wk and then transferring cultures to a 1 μM BA medium for 6 more wk increased the number of transferable shoots, but not significantly. However, our data predicts that the maximum number of transferable shoots produced from a single microshoot would occur on media with 5.4 μM zeatin riboside. Shoots rooted easily in vitro or ex vitro and rooted shoots were easily acclimatized. The methods described in this paper are being used to commercially micropropagate H. parviflora.",

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AU - Richwine, Arthur M.

AU - Tipton, Jimmy L.

AU - Thompson, Gary A.

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N2 - We successfully micropropagated Hesperaloe parviflora from mature plants. Shoot cultures were directly initiated from mature plants using pedicel bud explants on a modified Murashige and Skoog medium containing Nitsch and Nitseh vitamins and 1 μM zeatin riboside. Axillary shoot multiplication from established cultures was most responsive to changing concentrations of N6-benzyladenine (BA) with the greatest production on 6 μM BA. Growing shoots on a medium supplemented with 6 μM BA for 6 wk and then transferring cultures to a 1 μM BA medium for 6 more wk increased the number of transferable shoots, but not significantly. However, our data predicts that the maximum number of transferable shoots produced from a single microshoot would occur on media with 5.4 μM zeatin riboside. Shoots rooted easily in vitro or ex vitro and rooted shoots were easily acclimatized. The methods described in this paper are being used to commercially micropropagate H. parviflora.

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Micropropagation of Hesperaloe parviflora

Abstract

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