TY - JOUR
T1 - Microsatellite-centromere mapping in the zebrafish (danio rerio)
AU - Kauffman, Elizabeth J.
AU - Gestl, Erin E.
AU - Kim, Don J.
AU - Walker, Charline
AU - Hite, Jill M.
AU - Yan, Guang
AU - Rogan, Peter K.
AU - Johnson, Stephen L.
AU - Cheng, Keith
N1 - Funding Information:
We thank Monte Wester®eld, Chuck Kimmel, and colleagues who, during several visits to the Institute of Neurobiology, provided advice and zebra®sh strains that allowed us to establish a zebra®sh colony and to work out conditions for early pressure parthenogenesis. Gary Ostrander (Department of Zoology, Oklahoma State University) provided a home for our zebra®sh during our move to Penn State and advice during setup of our new lab. We also thank Clifford Tabin (Harvard) for microsatellite primers, John Postlethwait (University of Oregon) for pairwise mapping data, and Michael Chorney (Penn State College of Medicine) for helpful comments on the manuscript. This work was supported by the Jake Gittlen Cancer Research Fund and grants from the American Cancer Society (IRG-196 and JFRA-581), the Four Diamonds Fund, and the National Science Foundation (MCB-9317817) to K.C.C.
PY - 1995/11
Y1 - 1995/11
N2 - Ten (CA)nmicrosatellite simple sequence repeat (SSR) markers, 1, 2, 12, 14, 16, 18, 20, 22, 26, and 29, were used to show high chiasma interference and to determine centromere-marker map distances in the zebrafish (Danio rerio). Of these, SSR 12 exhibited no recombinant tetratypes among 175 half-tetrad embryos, placing this marker within 1 cM of the centromere of Linkage Group XVII. Fractions of heterozygous half-tetrads for the remaining nine markers ranged from 0.64 to 0.89. Of these, six recombinant fractions were more than 0.67 (P< 0.05), indicating strong chiasma interference during female meiosis in the zebrafish. Consistent with previous mapping data, SSRs 2 and 20 of Linkage Group VI were tightly linked. Half-tetrad analysis will allow the mapping of the remaining centromeres and may be useful in the mapping of new genes and mutations in the zebrafish.
AB - Ten (CA)nmicrosatellite simple sequence repeat (SSR) markers, 1, 2, 12, 14, 16, 18, 20, 22, 26, and 29, were used to show high chiasma interference and to determine centromere-marker map distances in the zebrafish (Danio rerio). Of these, SSR 12 exhibited no recombinant tetratypes among 175 half-tetrad embryos, placing this marker within 1 cM of the centromere of Linkage Group XVII. Fractions of heterozygous half-tetrads for the remaining nine markers ranged from 0.64 to 0.89. Of these, six recombinant fractions were more than 0.67 (P< 0.05), indicating strong chiasma interference during female meiosis in the zebrafish. Consistent with previous mapping data, SSRs 2 and 20 of Linkage Group VI were tightly linked. Half-tetrad analysis will allow the mapping of the remaining centromeres and may be useful in the mapping of new genes and mutations in the zebrafish.
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U2 - 10.1006/geno.1995.9869
DO - 10.1006/geno.1995.9869
M3 - Article
C2 - 8586435
AN - SCOPUS:0028829770
SN - 0888-7543
VL - 30
SP - 337
EP - 341
JO - Genomics
JF - Genomics
IS - 2
ER -