Microsatellite DNA markers for the study of horseshoe crab (Limulus polyphemus) population structure

Tim L. King; Michael S. Eackles

doi:10.1111/j.1471-8286.2004.00663.x

Microsatellite DNA markers for the study of horseshoe crab (Limulus polyphemus) population structure

Tim L. King, Michael S. Eackles

Ecosystem Science and Management

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19 Scopus citations

Abstract

Twenty-two microsatellite DNA loci were identified and characterized for horseshoe crabs (Limulus polyphemus) collected from two Atlantic coast and one Gulf of Mexico site. These markers revealed a high degree of genetic diversity (8-35 alleles per locus), heterozygosity (25.0% to 100.0%), and allelic heterogeneity (69.8% of comparisons). Considerable regional differentiation was observed as genetic distances (chord) ranged between 0.25 and 0.45, and all F_ST values (0.014-0.092) were significant. These preliminary findings are consistent with patterns of regional differentiation observed using allozyme variation and contradictory to findings of limited gene flow reported for sequence variation at the mitochondrial DNA COI region.

Original language	English (US)
Pages (from-to)	394-396
Number of pages	3
Journal	Molecular Ecology Notes
Volume	4
Issue number	3
DOIs	https://doi.org/10.1111/j.1471-8286.2004.00663.x
State	Published - Sep 2004

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology
Biochemistry
Ecology

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10.1111/j.1471-8286.2004.00663.x

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abstract = "Twenty-two microsatellite DNA loci were identified and characterized for horseshoe crabs (Limulus polyphemus) collected from two Atlantic coast and one Gulf of Mexico site. These markers revealed a high degree of genetic diversity (8-35 alleles per locus), heterozygosity (25.0% to 100.0%), and allelic heterogeneity (69.8% of comparisons). Considerable regional differentiation was observed as genetic distances (chord) ranged between 0.25 and 0.45, and all FST values (0.014-0.092) were significant. These preliminary findings are consistent with patterns of regional differentiation observed using allozyme variation and contradictory to findings of limited gene flow reported for sequence variation at the mitochondrial DNA COI region.",

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AB - Twenty-two microsatellite DNA loci were identified and characterized for horseshoe crabs (Limulus polyphemus) collected from two Atlantic coast and one Gulf of Mexico site. These markers revealed a high degree of genetic diversity (8-35 alleles per locus), heterozygosity (25.0% to 100.0%), and allelic heterogeneity (69.8% of comparisons). Considerable regional differentiation was observed as genetic distances (chord) ranged between 0.25 and 0.45, and all FST values (0.014-0.092) were significant. These preliminary findings are consistent with patterns of regional differentiation observed using allozyme variation and contradictory to findings of limited gene flow reported for sequence variation at the mitochondrial DNA COI region.

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Microsatellite DNA markers for the study of horseshoe crab (Limulus polyphemus) population structure

Abstract

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