Microsatellite loci for the host-generalist "zooxanthella" Symbiodinium trenchi and other Clade D Symbiodinium

Drew C. Wham; Daniel T. Pettay; Todd C. LaJeunesse

doi:10.1007/s12686-011-9399-2

Microsatellite loci for the host-generalist "zooxanthella" Symbiodinium trenchi and other Clade D Symbiodinium

Drew C. Wham, Daniel T. Pettay, Todd C. LaJeunesse

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Nine new polymorphic microsatellites were developed for Symbiodinium trenchi (sensu type D1a). These loci were tested on populations of S. trenchi from corals in Palau and 3-19 alleles were observed at each haploid locus with an average of 7 alleles. Many of the primer sets successfully amplified loci within other members of Symbiodinium clade D, demonstrating their utility across the group. Clade D Symbiodinium spp. are generally regarded as thermally tolerant and are common in coral reef habitats with warm, turbid, and/or variable environmental conditions. These population genetic markers are therefore useful for investigating how stress-tolerant symbionts may respond to climate warming through range expansion and/or by developing new associations with various host cnidarians. Additionally, the cross-lineage utility of these markers should help delineate the evolutionary relationships among members of this clade.

Original language	English (US)
Pages (from-to)	541-544
Number of pages	4
Journal	Conservation Genetics Resources
Volume	3
Issue number	3
DOIs	https://doi.org/10.1007/s12686-011-9399-2
State	Published - Jul 2011

All Science Journal Classification (ASJC) codes

Ecology, Evolution, Behavior and Systematics
Genetics

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10.1007/s12686-011-9399-2

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title = "Microsatellite loci for the host-generalist {"}zooxanthella{"} Symbiodinium trenchi and other Clade D Symbiodinium",

abstract = "Nine new polymorphic microsatellites were developed for Symbiodinium trenchi (sensu type D1a). These loci were tested on populations of S. trenchi from corals in Palau and 3-19 alleles were observed at each haploid locus with an average of 7 alleles. Many of the primer sets successfully amplified loci within other members of Symbiodinium clade D, demonstrating their utility across the group. Clade D Symbiodinium spp. are generally regarded as thermally tolerant and are common in coral reef habitats with warm, turbid, and/or variable environmental conditions. These population genetic markers are therefore useful for investigating how stress-tolerant symbionts may respond to climate warming through range expansion and/or by developing new associations with various host cnidarians. Additionally, the cross-lineage utility of these markers should help delineate the evolutionary relationships among members of this clade.",

author = "Wham, {Drew C.} and Pettay, {Daniel T.} and LaJeunesse, {Todd C.}",

note = "Funding Information: Acknowledgments We would like to thank Yi-T Lien and Allen Chen for providing samples of Symbiodinium from Japan and Dustin Kemp for helping collect samples in Palau. We would also like to thank Deb and Greg Grove and the Penn State Genomics Core Facility at University Park for generating the 454 sequence data used in this study. This research was funded in part by The Pennsylvania State University and the National Science Foundation (OCE-09287664) as well as the IOC-UNESCO-World Bank targeted working group on coral bleaching.",

year = "2011",

month = jul,

doi = "10.1007/s12686-011-9399-2",

language = "English (US)",

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pages = "541--544",

journal = "Conservation Genetics Resources",

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AU - Wham, Drew C.

AU - Pettay, Daniel T.

AU - LaJeunesse, Todd C.

N1 - Funding Information: Acknowledgments We would like to thank Yi-T Lien and Allen Chen for providing samples of Symbiodinium from Japan and Dustin Kemp for helping collect samples in Palau. We would also like to thank Deb and Greg Grove and the Penn State Genomics Core Facility at University Park for generating the 454 sequence data used in this study. This research was funded in part by The Pennsylvania State University and the National Science Foundation (OCE-09287664) as well as the IOC-UNESCO-World Bank targeted working group on coral bleaching.

PY - 2011/7

Y1 - 2011/7

N2 - Nine new polymorphic microsatellites were developed for Symbiodinium trenchi (sensu type D1a). These loci were tested on populations of S. trenchi from corals in Palau and 3-19 alleles were observed at each haploid locus with an average of 7 alleles. Many of the primer sets successfully amplified loci within other members of Symbiodinium clade D, demonstrating their utility across the group. Clade D Symbiodinium spp. are generally regarded as thermally tolerant and are common in coral reef habitats with warm, turbid, and/or variable environmental conditions. These population genetic markers are therefore useful for investigating how stress-tolerant symbionts may respond to climate warming through range expansion and/or by developing new associations with various host cnidarians. Additionally, the cross-lineage utility of these markers should help delineate the evolutionary relationships among members of this clade.

AB - Nine new polymorphic microsatellites were developed for Symbiodinium trenchi (sensu type D1a). These loci were tested on populations of S. trenchi from corals in Palau and 3-19 alleles were observed at each haploid locus with an average of 7 alleles. Many of the primer sets successfully amplified loci within other members of Symbiodinium clade D, demonstrating their utility across the group. Clade D Symbiodinium spp. are generally regarded as thermally tolerant and are common in coral reef habitats with warm, turbid, and/or variable environmental conditions. These population genetic markers are therefore useful for investigating how stress-tolerant symbionts may respond to climate warming through range expansion and/or by developing new associations with various host cnidarians. Additionally, the cross-lineage utility of these markers should help delineate the evolutionary relationships among members of this clade.

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Microsatellite loci for the host-generalist "zooxanthella" Symbiodinium trenchi and other Clade D Symbiodinium

Abstract

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