TY - JOUR
T1 - Microsatellite markers for Corybas (Orchidaceae) species in New Zealand
AU - Van Etten, Megan L.
AU - Lehnebach, Carlos A.
AU - Pearson, Sofie M.
AU - Robertson, Alastair W.
AU - Tate, Jennifer A.
N1 - Funding Information:
This project was supported by Marsden Fund MNZ1001 to C.A.L. and A.W.R. Samples were collected under New Zealand Department of Conservation and Greater Wellington City Council permits (BP-30352-FLO, TW-29987-FLO, 35026-FAU, WE-33250-FLO, and CA-29892-FLO). We thank Prashant Joshi for technical assistance.
Publisher Copyright:
© 2018 Van Etten et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America.
PY - 2018/11
Y1 - 2018/11
N2 - Premise of the Study: Microsatellite markers were developed for New Zealand species of Corybas (Orchidaceae) to investigate population genetics and species delimitation. Methods and Results: From sequencing a total genomic DNA library (using Illumina MiSeq), we developed 22 microsatellite markers for C. obscurus. The di- and trinucleotide repeat loci were initially trialed on individuals representing seven Corybas taxa (C. “rimutaka,” C. confusus, C. hypogaeus, C. macranthus, C. obscurus, C. trilobus, and C. walliae) and had one to eight alleles per locus. Twelve polymorphic markers were further tested on six Corybas populations from three of the seven taxa (C. obscurus, C. “rimutaka,” and C. trilobus). Observed and expected heterozygosities ranged from 0–1 and 0–0.859, respectively. The utility of these 12 loci was further validated in five related Corybas species (C. hypogaeus, C. obscurus, C. vitreus, C. walliae, and C. “rimutaka”; 38 individuals) representing populations from across the North and South Islands. The average value for genetic diversity among populations (FST) of 0.439 shows differentiation among species. Conclusions: These markers will be useful for future studies aimed at delimiting species boundaries and examining the genetic diversity of the New Zealand Corybas species.
AB - Premise of the Study: Microsatellite markers were developed for New Zealand species of Corybas (Orchidaceae) to investigate population genetics and species delimitation. Methods and Results: From sequencing a total genomic DNA library (using Illumina MiSeq), we developed 22 microsatellite markers for C. obscurus. The di- and trinucleotide repeat loci were initially trialed on individuals representing seven Corybas taxa (C. “rimutaka,” C. confusus, C. hypogaeus, C. macranthus, C. obscurus, C. trilobus, and C. walliae) and had one to eight alleles per locus. Twelve polymorphic markers were further tested on six Corybas populations from three of the seven taxa (C. obscurus, C. “rimutaka,” and C. trilobus). Observed and expected heterozygosities ranged from 0–1 and 0–0.859, respectively. The utility of these 12 loci was further validated in five related Corybas species (C. hypogaeus, C. obscurus, C. vitreus, C. walliae, and C. “rimutaka”; 38 individuals) representing populations from across the North and South Islands. The average value for genetic diversity among populations (FST) of 0.439 shows differentiation among species. Conclusions: These markers will be useful for future studies aimed at delimiting species boundaries and examining the genetic diversity of the New Zealand Corybas species.
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U2 - 10.1002/aps3.1192
DO - 10.1002/aps3.1192
M3 - Article
C2 - 30473938
AN - SCOPUS:85056351537
SN - 2168-0450
VL - 6
JO - Applications in Plant Sciences
JF - Applications in Plant Sciences
IS - 11
M1 - e01192
ER -