TY - JOUR
T1 - Microsclerotia development in Verticillium dahliae
T2 - Regulation and differential expression of the hydrophobin gene VDH1
AU - Klimes, Anna
AU - Amyotte, Stefan G.
AU - Grant, Sandra
AU - Kang, Seogchan
AU - Dobinson, Katherine F.
N1 - Funding Information:
This research was funded in part by a Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant to K.F.D. Work by S.K. was supported by a grant from USDA-NRI (2002-02367). A.K. was a recipient of an Ontario Graduate Scholarship in Science and Technology. We thank A. Molnar for graphics support.
PY - 2008/12
Y1 - 2008/12
N2 - The vascular wilt fungus Verticillium dahliae produces persistent resting structures, known as microsclerotia, which are important for this plant pathogen's long-term survival. Previously, we identified a hydrophobin gene (VDH1) that is necessary for microsclerotial production. The current study of VDH1's expression, and its regulation, was undertaken to provide insight into the largely uncharacterized molecular mechanisms relevant to microsclerotial development. Reporter gene analysis showed that VDH1 is specifically expressed in developing microsclerotia, as well as in hyphal fusions and conidiophores, suggesting that VDH1 mediates the development of microsclerotia from conidiophores and other hyphal structures. We report also on the effects of nutrient availability on the regulation of microsclerotial development in V. dahliae; the gene's activity appears to be regulated in response to carbon availability. Lastly, constitutive expression of VDH1 results in delayed disease symptom development, but has no noticeable effect on in vitro microsclerotial development. Crown
AB - The vascular wilt fungus Verticillium dahliae produces persistent resting structures, known as microsclerotia, which are important for this plant pathogen's long-term survival. Previously, we identified a hydrophobin gene (VDH1) that is necessary for microsclerotial production. The current study of VDH1's expression, and its regulation, was undertaken to provide insight into the largely uncharacterized molecular mechanisms relevant to microsclerotial development. Reporter gene analysis showed that VDH1 is specifically expressed in developing microsclerotia, as well as in hyphal fusions and conidiophores, suggesting that VDH1 mediates the development of microsclerotia from conidiophores and other hyphal structures. We report also on the effects of nutrient availability on the regulation of microsclerotial development in V. dahliae; the gene's activity appears to be regulated in response to carbon availability. Lastly, constitutive expression of VDH1 results in delayed disease symptom development, but has no noticeable effect on in vitro microsclerotial development. Crown
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U2 - 10.1016/j.fgb.2008.09.014
DO - 10.1016/j.fgb.2008.09.014
M3 - Article
C2 - 18951989
AN - SCOPUS:56049089368
SN - 1087-1845
VL - 45
SP - 1525
EP - 1532
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
IS - 12
ER -