Miniature postsynaptic currents depend on Ca2+ released from internal stores via PLC/IP3 pathway

M. H. Han; A. Kawasaki; J. Y. Wei; C. J. Barnstable

doi:10.1097/00001756-200107200-00032

Miniature postsynaptic currents depend on Ca²⁺ released from internal stores via PLC/IP₃ pathway

M. H. Han, A. Kawasaki, J. Y. Wei, C. J. Barnstable

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Miniature postsynaptic currents (mPSCs) were examined on autaptic innervation of single rat retinal ganglion cells in low density cultures. Removal of Ca²⁺ from bath solution or blocking of Ca²⁺ channels by Cd²⁺ had no detectable effect on mPSC frequency or amplitude. Thapsigargin, an agent for mobilization of Ca²⁺ from internal stores, increased mPSC frequency 3-5-fold in control, Ca²⁺-free or Cd²⁺-containing solutions. The inositol 1,4,5-triphosphate (IP₃) receptor antagonist, heparin; the phospholipase C (PLC) inhibitor, U73122; and caffeine abolished mPSC or decreased mPSCs frequency. Calcium imaging showed that cytosolic Ca²⁺ was increased by thapsigargin and decreased by caffeine. These data demonstrate that internal store-released Ca²⁺ regulated by the PLC/IP₃/IP₃-receptor pathway has critical contribution to generation and control of miniature release in retinal ganglion cells.

Original language	English (US)
Pages (from-to)	2203-2207
Number of pages	5
Journal	Neuroreport
Volume	12
Issue number	10
DOIs	https://doi.org/10.1097/00001756-200107200-00032
State	Published - Jul 20 2001

All Science Journal Classification (ASJC) codes

General Neuroscience

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10.1097/00001756-200107200-00032

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abstract = "Miniature postsynaptic currents (mPSCs) were examined on autaptic innervation of single rat retinal ganglion cells in low density cultures. Removal of Ca2+ from bath solution or blocking of Ca2+ channels by Cd2+ had no detectable effect on mPSC frequency or amplitude. Thapsigargin, an agent for mobilization of Ca2+ from internal stores, increased mPSC frequency 3-5-fold in control, Ca2+-free or Cd2+-containing solutions. The inositol 1,4,5-triphosphate (IP3) receptor antagonist, heparin; the phospholipase C (PLC) inhibitor, U73122; and caffeine abolished mPSC or decreased mPSCs frequency. Calcium imaging showed that cytosolic Ca2+ was increased by thapsigargin and decreased by caffeine. These data demonstrate that internal store-released Ca2+ regulated by the PLC/IP3/IP3-receptor pathway has critical contribution to generation and control of miniature release in retinal ganglion cells.",

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AU - Han, M. H.

AU - Kawasaki, A.

AU - Wei, J. Y.

AU - Barnstable, C. J.

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N2 - Miniature postsynaptic currents (mPSCs) were examined on autaptic innervation of single rat retinal ganglion cells in low density cultures. Removal of Ca2+ from bath solution or blocking of Ca2+ channels by Cd2+ had no detectable effect on mPSC frequency or amplitude. Thapsigargin, an agent for mobilization of Ca2+ from internal stores, increased mPSC frequency 3-5-fold in control, Ca2+-free or Cd2+-containing solutions. The inositol 1,4,5-triphosphate (IP3) receptor antagonist, heparin; the phospholipase C (PLC) inhibitor, U73122; and caffeine abolished mPSC or decreased mPSCs frequency. Calcium imaging showed that cytosolic Ca2+ was increased by thapsigargin and decreased by caffeine. These data demonstrate that internal store-released Ca2+ regulated by the PLC/IP3/IP3-receptor pathway has critical contribution to generation and control of miniature release in retinal ganglion cells.

AB - Miniature postsynaptic currents (mPSCs) were examined on autaptic innervation of single rat retinal ganglion cells in low density cultures. Removal of Ca2+ from bath solution or blocking of Ca2+ channels by Cd2+ had no detectable effect on mPSC frequency or amplitude. Thapsigargin, an agent for mobilization of Ca2+ from internal stores, increased mPSC frequency 3-5-fold in control, Ca2+-free or Cd2+-containing solutions. The inositol 1,4,5-triphosphate (IP3) receptor antagonist, heparin; the phospholipase C (PLC) inhibitor, U73122; and caffeine abolished mPSC or decreased mPSCs frequency. Calcium imaging showed that cytosolic Ca2+ was increased by thapsigargin and decreased by caffeine. These data demonstrate that internal store-released Ca2+ regulated by the PLC/IP3/IP3-receptor pathway has critical contribution to generation and control of miniature release in retinal ganglion cells.

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Miniature postsynaptic currents depend on Ca²⁺ released from internal stores via PLC/IP₃ pathway

Abstract

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