TY - JOUR
T1 - Mining new microsatellite markers for Siberian apricot (Prunus sibirica L.) from SSR-enriched genomic library
AU - Wang, Zhe
AU - Liu, Huabo
AU - Liu, Jun
AU - Li, Yingyue
AU - Wu, Rongling
AU - Pang, Xiaoming
N1 - Funding Information:
This work was supported by grants from the Program for New Century Excellent Talents in University by Ministry of Education, China ( NCET-10-0223 ), the Starting funds from the Office for Science and Technology of Beijing Forestry University, China ( 2008-03 ) to Dr. Xiaoming Pang and the Changjiang Scholars Award and “Thousand-person Plan” Award to Dr. Rongling Wu. We thank to Dr. Yunqian Guo of Beijing Forestry University for her valuable critical modifying the manuscript.
PY - 2014/2/13
Y1 - 2014/2/13
N2 - Siberian apricot (Prunus sibirica L.) is a multipurpose tree species of great economic and ecological importance. It displays abundant natural variation which provides a fuel for it to adapt to various environments. The development of polymorphic markers has become increasingly essential for quantifying the genetic diversity of this species and charactering quantitative trait loci (QTLs) that control complex traits. SSR markers are the most suitable molecular markers owning to their desirable genetic attributes. However, the number of SSR makers developed for Siberian apricot is limited. Thus in this report, using magnetic beads enrichment protocol, we obtained 143 unique sequences from two GA-enriched genomic libraries with two blunt end-cutting restriction enzymes (AluI, HaeIII) and successfully developed 52 primer pairs. Among them, 46 loci showed polymorphism in eight P. sibirica accessions and the number of alleles obtained ranged from 2 to 12 with an average of 6.20. The polymorphism information content (PIC) for the polymorphic SSRs ranged from 0.11 to 0.90 with an average of 0.62. The present study provides valuable tools for genetic studies in Siberian apricot and related species, such as population genetics, germplasm identification, QTL mapping and marker assisted selection.
AB - Siberian apricot (Prunus sibirica L.) is a multipurpose tree species of great economic and ecological importance. It displays abundant natural variation which provides a fuel for it to adapt to various environments. The development of polymorphic markers has become increasingly essential for quantifying the genetic diversity of this species and charactering quantitative trait loci (QTLs) that control complex traits. SSR markers are the most suitable molecular markers owning to their desirable genetic attributes. However, the number of SSR makers developed for Siberian apricot is limited. Thus in this report, using magnetic beads enrichment protocol, we obtained 143 unique sequences from two GA-enriched genomic libraries with two blunt end-cutting restriction enzymes (AluI, HaeIII) and successfully developed 52 primer pairs. Among them, 46 loci showed polymorphism in eight P. sibirica accessions and the number of alleles obtained ranged from 2 to 12 with an average of 6.20. The polymorphism information content (PIC) for the polymorphic SSRs ranged from 0.11 to 0.90 with an average of 0.62. The present study provides valuable tools for genetic studies in Siberian apricot and related species, such as population genetics, germplasm identification, QTL mapping and marker assisted selection.
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U2 - 10.1016/j.scienta.2013.12.004
DO - 10.1016/j.scienta.2013.12.004
M3 - Article
AN - SCOPUS:84891387578
SN - 0304-4238
VL - 166
SP - 65
EP - 69
JO - Scientia Horticulturae
JF - Scientia Horticulturae
ER -