TY - JOUR
T1 - Mitochondria impairment correlates with increased sensitivity of aging RPE cells to oxidative stress
AU - He, Yuan
AU - Ge, Jian
AU - Burke, Janice M.
AU - Myers, Roland L.
AU - Dong, Zhi Z.
AU - Tombran-Tink, Joyce
N1 - Funding Information:
Acknowledgments This work was supported by grants from the Ben Franklin Foundation, PA, USA, the National Basic Research Program of China (no. 2007CB512200), and the National Natural Science Foundation of China (no. 30672275, no. 30400486), NEI Core Grant P30 EY01931, and an unrestricted grant from Research to Prevent Blindness, Inc. to the Medical College of Wisconsin, and by grants from Shaanxi Province Departments of Health (no. 2010D56) and Education (no. 2010JK807), China. The first author received support from the China Scholarship Council.
PY - 2010/9
Y1 - 2010/9
N2 - Impairment of mitochondria function and cellular antioxidant systems are linked to aging and neurodegenerative diseases. In the eye, the retinal pigment epithelium (RPE) is exposed to a highly oxidative environment that contributes to age-related visual dysfunction. Here, we examined changes in mitochondrial function in human RPE cells and sensitivity to oxidative stress with increased chronological age. Primary RPE cells from young (9-20)-, mid-age (48-60)-, and g̃;60 (62-76)-year-old donors were grown to confluency and examined by electron microscopy and flow cytometry using several mitochondrial functional assessment tools. Susceptibility of RPE cells to H2O2 toxicity was determined by lactate dehydrogenase and cytochrome c release, as well as propidium iodide staining. Reactive oxygen species, cytoplasmic Ca 2+ [Ca2+]c, and mitochondrial Ca2+ [Ca2+]m levels were measured using
AB - Impairment of mitochondria function and cellular antioxidant systems are linked to aging and neurodegenerative diseases. In the eye, the retinal pigment epithelium (RPE) is exposed to a highly oxidative environment that contributes to age-related visual dysfunction. Here, we examined changes in mitochondrial function in human RPE cells and sensitivity to oxidative stress with increased chronological age. Primary RPE cells from young (9-20)-, mid-age (48-60)-, and g̃;60 (62-76)-year-old donors were grown to confluency and examined by electron microscopy and flow cytometry using several mitochondrial functional assessment tools. Susceptibility of RPE cells to H2O2 toxicity was determined by lactate dehydrogenase and cytochrome c release, as well as propidium iodide staining. Reactive oxygen species, cytoplasmic Ca 2+ [Ca2+]c, and mitochondrial Ca2+ [Ca2+]m levels were measured using
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U2 - 10.1007/s12177-011-9061-y
DO - 10.1007/s12177-011-9061-y
M3 - Article
C2 - 22833778
AN - SCOPUS:80055003440
SN - 1936-8437
VL - 3
SP - 92
EP - 108
JO - Journal of Ocular Biology, Diseases, and Informatics
JF - Journal of Ocular Biology, Diseases, and Informatics
IS - 3
ER -
