Mitochondria impairment correlates with increased sensitivity of aging RPE cells to oxidative stress

Yuan He; Jian Ge; Janice M. Burke; Roland L. Myers; Zhi Z. Dong; Joyce Tombran-Tink

doi:10.1007/s12177-011-9061-y

Mitochondria impairment correlates with increased sensitivity of aging RPE cells to oxidative stress

Yuan He
, Jian Ge
, Janice M. Burke
, Roland L. Myers
, Zhi Z. Dong
, Joyce Tombran-Tink

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Impairment of mitochondria function and cellular antioxidant systems are linked to aging and neurodegenerative diseases. In the eye, the retinal pigment epithelium (RPE) is exposed to a highly oxidative environment that contributes to age-related visual dysfunction. Here, we examined changes in mitochondrial function in human RPE cells and sensitivity to oxidative stress with increased chronological age. Primary RPE cells from young (9-20)-, mid-age (48-60)-, and g̃;60 (62-76)-year-old donors were grown to confluency and examined by electron microscopy and flow cytometry using several mitochondrial functional assessment tools. Susceptibility of RPE cells to H₂O₂ toxicity was determined by lactate dehydrogenase and cytochrome c release, as well as propidium iodide staining. Reactive oxygen species, cytoplasmic Ca ²⁺ [Ca²⁺]_c, and mitochondrial Ca²⁺ [Ca²⁺]_m levels were measured using

Original language	English (US)
Pages (from-to)	92-108
Number of pages	17
Journal	Journal of Ocular Biology, Diseases, and Informatics
Volume	3
Issue number	3
DOIs	https://doi.org/10.1007/s12177-011-9061-y
State	Published - Sep 2010

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Ophthalmology
Genetics

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10.1007/s12177-011-9061-y

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title = "Mitochondria impairment correlates with increased sensitivity of aging RPE cells to oxidative stress",

abstract = "Impairment of mitochondria function and cellular antioxidant systems are linked to aging and neurodegenerative diseases. In the eye, the retinal pigment epithelium (RPE) is exposed to a highly oxidative environment that contributes to age-related visual dysfunction. Here, we examined changes in mitochondrial function in human RPE cells and sensitivity to oxidative stress with increased chronological age. Primary RPE cells from young (9-20)-, mid-age (48-60)-, and {\~g};60 (62-76)-year-old donors were grown to confluency and examined by electron microscopy and flow cytometry using several mitochondrial functional assessment tools. Susceptibility of RPE cells to H2O2 toxicity was determined by lactate dehydrogenase and cytochrome c release, as well as propidium iodide staining. Reactive oxygen species, cytoplasmic Ca 2+ [Ca2+]c, and mitochondrial Ca2+ [Ca2+]m levels were measured using",

author = "Yuan He and Jian Ge and Burke, \{Janice M.\} and Myers, \{Roland L.\} and Dong, \{Zhi Z.\} and Joyce Tombran-Tink",

note = "Funding Information: Acknowledgments This work was supported by grants from the Ben Franklin Foundation, PA, USA, the National Basic Research Program of China (no. 2007CB512200), and the National Natural Science Foundation of China (no. 30672275, no. 30400486), NEI Core Grant P30 EY01931, and an unrestricted grant from Research to Prevent Blindness, Inc. to the Medical College of Wisconsin, and by grants from Shaanxi Province Departments of Health (no. 2010D56) and Education (no. 2010JK807), China. The first author received support from the China Scholarship Council.",

year = "2010",

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doi = "10.1007/s12177-011-9061-y",

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AU - Myers, Roland L.

AU - Dong, Zhi Z.

AU - Tombran-Tink, Joyce

N1 - Funding Information: Acknowledgments This work was supported by grants from the Ben Franklin Foundation, PA, USA, the National Basic Research Program of China (no. 2007CB512200), and the National Natural Science Foundation of China (no. 30672275, no. 30400486), NEI Core Grant P30 EY01931, and an unrestricted grant from Research to Prevent Blindness, Inc. to the Medical College of Wisconsin, and by grants from Shaanxi Province Departments of Health (no. 2010D56) and Education (no. 2010JK807), China. The first author received support from the China Scholarship Council.

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Mitochondria impairment correlates with increased sensitivity of aging RPE cells to oxidative stress

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