TY - JOUR
T1 - Mitochondrial DNA from lupine
T2 - restriction analysis and cloning of fragments coding for tRNA
AU - Augustyniak, Halina
AU - Borsuk, Piotr
AU - Hirschler-Laszkiewicz, Iwona
AU - Stepień, Piotr P.
AU - Bartnik, Ewa
N1 - Funding Information:
Pawelkiewicz for his interest. This work was supported by the Polish Academy of Sciences within Project 09.7.
PY - 1983/4
Y1 - 1983/4
N2 - Mitochondrial DNA (mtDNA) was isolated from lupine. Restriction analysis was used to estimate its size, which is about 180 kb. A BamHI bank of this mtDNA was constructed using plasmids pBR322 and pBR327 as vectors. Eight clones containing plasmids hybridizing to mitochondrial tRNA (mttRNA) were isolated. Restriction maps of these plasmids were determined. Six of these plasmids hybridized to unique fragments and two to two fragments of very similar size, all obtained by BamHI cleavage of mtDNA.
AB - Mitochondrial DNA (mtDNA) was isolated from lupine. Restriction analysis was used to estimate its size, which is about 180 kb. A BamHI bank of this mtDNA was constructed using plasmids pBR322 and pBR327 as vectors. Eight clones containing plasmids hybridizing to mitochondrial tRNA (mttRNA) were isolated. Restriction maps of these plasmids were determined. Six of these plasmids hybridized to unique fragments and two to two fragments of very similar size, all obtained by BamHI cleavage of mtDNA.
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U2 - 10.1016/0378-1119(83)90065-3
DO - 10.1016/0378-1119(83)90065-3
M3 - Article
C2 - 6305772
AN - SCOPUS:0020581995
SN - 0378-1119
VL - 22
SP - 69
EP - 74
JO - Gene
JF - Gene
IS - 1
ER -