TY - GEN
T1 - Mobile all-in-one malaria molecular diagnosis for field deployment in resource-limited areas
AU - Choi, Gihoon
AU - Song, Daniel
AU - Miao, Jun
AU - Cui, Liwang
AU - Guan, Weihua
N1 - Publisher Copyright:
© 2016 IEEE.
PY - 2016/12/27
Y1 - 2016/12/27
N2 - This paper presents a 'sample-in-answer-out' molecular diagnostic system (AnyMDx) for blood-born malaria detection at the point of care, especially in resource-limiting settings. AnyMDx consists of a microfluidic reagent compact disk incorporating seamlessly integrated steps of DNA extraction, purification, elution, and amplification, as well as a mobile battery-powered analyzer. Low power thermal module and novel fluorescence sensing module are integrated into the analyzer for real-time monitoring of loop-mediated isothermal nucleic acid amplification (LAMP). The AnyMDx has an assay detection limit of ∼0.6 parasites/μL against blood P. falciparum parasites, much lower than conventional microscope-based method (∼50 parasites/μL) and immunoassay based RTD (∼100 parasites/μL). AnyMDx is fully automated and thus is easily accessible to non-specialist with minimal molecular training. The sample-to-answer turnaround time for AnyMDx is less than 40 minutes.
AB - This paper presents a 'sample-in-answer-out' molecular diagnostic system (AnyMDx) for blood-born malaria detection at the point of care, especially in resource-limiting settings. AnyMDx consists of a microfluidic reagent compact disk incorporating seamlessly integrated steps of DNA extraction, purification, elution, and amplification, as well as a mobile battery-powered analyzer. Low power thermal module and novel fluorescence sensing module are integrated into the analyzer for real-time monitoring of loop-mediated isothermal nucleic acid amplification (LAMP). The AnyMDx has an assay detection limit of ∼0.6 parasites/μL against blood P. falciparum parasites, much lower than conventional microscope-based method (∼50 parasites/μL) and immunoassay based RTD (∼100 parasites/μL). AnyMDx is fully automated and thus is easily accessible to non-specialist with minimal molecular training. The sample-to-answer turnaround time for AnyMDx is less than 40 minutes.
UR - http://www.scopus.com/inward/record.url?scp=85010670094&partnerID=8YFLogxK
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U2 - 10.1109/HIC.2016.7797734
DO - 10.1109/HIC.2016.7797734
M3 - Conference contribution
AN - SCOPUS:85010670094
T3 - 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
SP - 212
EP - 215
BT - 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
Y2 - 9 November 2016 through 11 November 2016
ER -