Mobile all-in-one malaria molecular diagnosis for field deployment in resource-limited areas

Gihoon Choi; Daniel Song; Jun Miao; Liwang Cui; Weihua Guan

doi:10.1109/HIC.2016.7797734

Mobile all-in-one malaria molecular diagnosis for field deployment in resource-limited areas

Gihoon Choi
, Daniel Song
, Jun Miao
, Liwang Cui
, Weihua Guan

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Scopus citations

Abstract

This paper presents a 'sample-in-answer-out' molecular diagnostic system (AnyMDx) for blood-born malaria detection at the point of care, especially in resource-limiting settings. AnyMDx consists of a microfluidic reagent compact disk incorporating seamlessly integrated steps of DNA extraction, purification, elution, and amplification, as well as a mobile battery-powered analyzer. Low power thermal module and novel fluorescence sensing module are integrated into the analyzer for real-time monitoring of loop-mediated isothermal nucleic acid amplification (LAMP). The AnyMDx has an assay detection limit of ∼0.6 parasites/μL against blood P. falciparum parasites, much lower than conventional microscope-based method (∼50 parasites/μL) and immunoassay based RTD (∼100 parasites/μL). AnyMDx is fully automated and thus is easily accessible to non-specialist with minimal molecular training. The sample-to-answer turnaround time for AnyMDx is less than 40 minutes.

Original language	English (US)
Title of host publication	2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	212-215
Number of pages	4
ISBN (Electronic)	9781509011667
DOIs	https://doi.org/10.1109/HIC.2016.7797734
State	Published - Dec 27 2016
Event	2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016 - Cancun, Mexico Duration: Nov 9 2016 → Nov 11 2016

Publication series

Name	2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016

Other

Other	2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
Country/Territory	Mexico
City	Cancun
Period	11/9/16 → 11/11/16

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Health Informatics
Medicine (miscellaneous)

Access to Document

10.1109/HIC.2016.7797734

Cite this

Choi, G., Song, D., Miao, J., Cui, L., & Guan, W. (2016). Mobile all-in-one malaria molecular diagnosis for field deployment in resource-limited areas. In 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016 (pp. 212-215). Article 7797734 (2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/HIC.2016.7797734

Choi, Gihoon ; Song, Daniel ; Miao, Jun et al. / Mobile all-in-one malaria molecular diagnosis for field deployment in resource-limited areas. 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016. Institute of Electrical and Electronics Engineers Inc., 2016. pp. 212-215 (2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016).

@inproceedings{50211d2cd3894cbdb955875cf353dd56,

title = "Mobile all-in-one malaria molecular diagnosis for field deployment in resource-limited areas",

abstract = "This paper presents a 'sample-in-answer-out' molecular diagnostic system (AnyMDx) for blood-born malaria detection at the point of care, especially in resource-limiting settings. AnyMDx consists of a microfluidic reagent compact disk incorporating seamlessly integrated steps of DNA extraction, purification, elution, and amplification, as well as a mobile battery-powered analyzer. Low power thermal module and novel fluorescence sensing module are integrated into the analyzer for real-time monitoring of loop-mediated isothermal nucleic acid amplification (LAMP). The AnyMDx has an assay detection limit of ∼0.6 parasites/μL against blood P. falciparum parasites, much lower than conventional microscope-based method (∼50 parasites/μL) and immunoassay based RTD (∼100 parasites/μL). AnyMDx is fully automated and thus is easily accessible to non-specialist with minimal molecular training. The sample-to-answer turnaround time for AnyMDx is less than 40 minutes.",

author = "Gihoon Choi and Daniel Song and Jun Miao and Liwang Cui and Weihua Guan",

note = "Publisher Copyright: {\textcopyright} 2016 IEEE.; 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016 ; Conference date: 09-11-2016 Through 11-11-2016",

year = "2016",

month = dec,

day = "27",

doi = "10.1109/HIC.2016.7797734",

language = "English (US)",

series = "2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "212--215",

booktitle = "2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016",

address = "United States",

}

Choi, G, Song, D, Miao, J, Cui, L & Guan, W 2016, Mobile all-in-one malaria molecular diagnosis for field deployment in resource-limited areas. in 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016., 7797734, 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016, Institute of Electrical and Electronics Engineers Inc., pp. 212-215, 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016, Cancun, Mexico, 11/9/16. https://doi.org/10.1109/HIC.2016.7797734

Mobile all-in-one malaria molecular diagnosis for field deployment in resource-limited areas. / Choi, Gihoon; Song, Daniel; Miao, Jun et al.
2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016. Institute of Electrical and Electronics Engineers Inc., 2016. p. 212-215 7797734 (2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Mobile all-in-one malaria molecular diagnosis for field deployment in resource-limited areas

AU - Choi, Gihoon

AU - Song, Daniel

AU - Miao, Jun

AU - Cui, Liwang

AU - Guan, Weihua

PY - 2016/12/27

Y1 - 2016/12/27

N2 - This paper presents a 'sample-in-answer-out' molecular diagnostic system (AnyMDx) for blood-born malaria detection at the point of care, especially in resource-limiting settings. AnyMDx consists of a microfluidic reagent compact disk incorporating seamlessly integrated steps of DNA extraction, purification, elution, and amplification, as well as a mobile battery-powered analyzer. Low power thermal module and novel fluorescence sensing module are integrated into the analyzer for real-time monitoring of loop-mediated isothermal nucleic acid amplification (LAMP). The AnyMDx has an assay detection limit of ∼0.6 parasites/μL against blood P. falciparum parasites, much lower than conventional microscope-based method (∼50 parasites/μL) and immunoassay based RTD (∼100 parasites/μL). AnyMDx is fully automated and thus is easily accessible to non-specialist with minimal molecular training. The sample-to-answer turnaround time for AnyMDx is less than 40 minutes.

AB - This paper presents a 'sample-in-answer-out' molecular diagnostic system (AnyMDx) for blood-born malaria detection at the point of care, especially in resource-limiting settings. AnyMDx consists of a microfluidic reagent compact disk incorporating seamlessly integrated steps of DNA extraction, purification, elution, and amplification, as well as a mobile battery-powered analyzer. Low power thermal module and novel fluorescence sensing module are integrated into the analyzer for real-time monitoring of loop-mediated isothermal nucleic acid amplification (LAMP). The AnyMDx has an assay detection limit of ∼0.6 parasites/μL against blood P. falciparum parasites, much lower than conventional microscope-based method (∼50 parasites/μL) and immunoassay based RTD (∼100 parasites/μL). AnyMDx is fully automated and thus is easily accessible to non-specialist with minimal molecular training. The sample-to-answer turnaround time for AnyMDx is less than 40 minutes.

UR - https://www.scopus.com/pages/publications/85010670094

UR - https://www.scopus.com/pages/publications/85010670094#tab=citedBy

U2 - 10.1109/HIC.2016.7797734

DO - 10.1109/HIC.2016.7797734

M3 - Conference contribution

AN - SCOPUS:85010670094

T3 - 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016

SP - 212

EP - 215

BT - 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016

Y2 - 9 November 2016 through 11 November 2016

ER -

Choi G, Song D, Miao J, Cui L, Guan W. Mobile all-in-one malaria molecular diagnosis for field deployment in resource-limited areas. In 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016. Institute of Electrical and Electronics Engineers Inc. 2016. p. 212-215. 7797734. (2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016). doi: 10.1109/HIC.2016.7797734

Mobile all-in-one malaria molecular diagnosis for field deployment in resource-limited areas

Abstract

Publication series

Other

UN SDGs

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this