Model multilayer structures for three-dimensional cell imaging

Joseph Kozole, Christopher Szakal, Michael Kurczy, Nicholas Winograd

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


The prospects for SIMS three-dimensional analysis of biological materials were explored using model multilayer structures. The samples were analyzed in a ToF-SIMS spectrometer equipped with a 20 keV buckminsterfullerene (C 60 + ) ion source. Molecular depth information was acquired using a C 60 + ion beam to etch through the multilayer structures at specified time intervals. Subsequent to each individual erosion cycle, static SIMS spectra were recorded using a pulsed C 60 + ion probe. Molecular intensities in sequential mass spectra were monitored as a function of primary ion fluence. The resulting depth information was used to characterize C 60 + bombardment of biological materials. Specifically, molecular depth profile studies involving dehydrated dipalmitoyl-phosphatidylcholine (DPPC) organic films indicate that cell membrane lipid materials do not experience significant chemical damage when bombarded with C 60 + ion fluences greater than 10 15 ions/cm 2 . Moreover, depth profile analyses of DPPC-sucrose frozen multilayer structures suggest that biomolecule information can be uncovered after the C 60 + sputter removal of a 20 nm overlayer with no appreciable loss of underlying molecular signal. The experimental results support the potential for three-dimensional molecular mapping of biological materials using cluster SIMS.

Original languageEnglish (US)
Pages (from-to)6789-6792
Number of pages4
JournalApplied Surface Science
Issue number19
StatePublished - Jul 30 2006

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • Condensed Matter Physics
  • General Physics and Astronomy
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films


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