TY - JOUR
T1 - Modulation of dihydropyridine receptors in vascular smooth muscle cells by membrane potential and cell proliferation
AU - Ruiz-Velasco, Victor
AU - Mayer, M. Beatriz
AU - Inscho, Edward W.
AU - Hymel, Lin J.
PY - 1994/8/16
Y1 - 1994/8/16
N2 - We have studied binding of isradipine to A7r5 vascular smooth muscle cells as a function of membrane potential and cell proliferation. Consistent with a voltage-modulated receptor model, two classes of binding sites were detected in confluent cultures: high-affinity sites under depolarizing (50 mM K+) conditions (Kd = 45 ± 3 pM), and lower affinity sites under resting (5 mM K+) conditions (Kd = 181 ± 20 pM). However, proliferating cells also displayed the high-affinity state at rest (Kd = 29 ± 9 pM) in addition to a low-affinity site (Kd = 869 ± 383 pM). Analysis of dissociation rates also revealed two receptor classes during proliferation. Proliferating cells showed a single class of high-affinity sites (Kd = 39 ± 6 pM) when depolarized, similar to confluent cells. Receptor density in confluent monolayers increased from 15 ± 3 fmol/106 cells at 5 days to 72 ± 6 fmol/106 cells after 10 days. These results suggest (i) that some L-type Ca2+ channels are spontaneously active in proliferating vascular smooth muscle cells, but require depolarization to activate in a confluent monolayer, and (ii) that the density of dihydropyridine receptors increases after a monolayer becomes confluent.
AB - We have studied binding of isradipine to A7r5 vascular smooth muscle cells as a function of membrane potential and cell proliferation. Consistent with a voltage-modulated receptor model, two classes of binding sites were detected in confluent cultures: high-affinity sites under depolarizing (50 mM K+) conditions (Kd = 45 ± 3 pM), and lower affinity sites under resting (5 mM K+) conditions (Kd = 181 ± 20 pM). However, proliferating cells also displayed the high-affinity state at rest (Kd = 29 ± 9 pM) in addition to a low-affinity site (Kd = 869 ± 383 pM). Analysis of dissociation rates also revealed two receptor classes during proliferation. Proliferating cells showed a single class of high-affinity sites (Kd = 39 ± 6 pM) when depolarized, similar to confluent cells. Receptor density in confluent monolayers increased from 15 ± 3 fmol/106 cells at 5 days to 72 ± 6 fmol/106 cells after 10 days. These results suggest (i) that some L-type Ca2+ channels are spontaneously active in proliferating vascular smooth muscle cells, but require depolarization to activate in a confluent monolayer, and (ii) that the density of dihydropyridine receptors increases after a monolayer becomes confluent.
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U2 - 10.1016/0922-4106(94)90055-8
DO - 10.1016/0922-4106(94)90055-8
M3 - Article
C2 - 7805757
AN - SCOPUS:0027981189
SN - 0922-4106
VL - 268
SP - 311
EP - 318
JO - European Journal of Pharmacology: Molecular Pharmacology
JF - European Journal of Pharmacology: Molecular Pharmacology
IS - 3
ER -