Molecular and cytological features of the mouse B-cell lymphoma line iMyc--1

Seong Su Han, Arthur L. Shaffer, Liangping Peng, Seung Tae Chung, Jae Hwan Lim, Sungho Maeng, Joong Su Kim, Nicole McNeil, Thomas Ried, Louis M. Staudt, Siegfried Janz

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Background: Myc-induced lymphoblastic B-cell lymphoma (LBL) in iMyc mice may provide a model system for the study of the mechanism by which human MYC facilitates the initiation and progression of B cell and plasma cell neoplasms in human beings. We have recently shown that gene-targeted iMyc mice that carry a His6-tagged mouse Myc cDNA, MycHis, just 5′ of the immunoglobulin heavy-chain enhancer, Eμ, are prone to B cell and plasma cell tumors. The predominant tumor (∼50%) that arose in the iMyc mice on the mixed genetic background of segregating C57BL/6 and 129/SvJ alleles was LBL. The purpose of this study was to establish and characterize a cell line, designated iMyc-1, for the in-depth evaluation of LBL in vitro. Methods: The morphological features and the surface marker expression profile of the iMyc-1 cells were evaluated using cytological methods and FACS, respectively. The cytogenetic make-up of the iMyc-1 cells was assessed by spectral karyotyping (SKY). The expression of the inserted MycHis gene was determined using RT-PCR and qPCR. Clonotypic immunoglobulin gene arrangements were detected by Southern blotting. The global gene expression program of the iMyc-1 cells and the expression of 768 "pathway" genes were determined with the help of the Mouse Lymphochip

Original languageEnglish (US)
Article number40
JournalMolecular Cancer
StatePublished - Nov 9 2005

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Oncology
  • Cancer Research


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